Ablashi D V, Lapps W, Kaplan M, Whitman J E, Richert J R, Pearson G R
Advanced Biotechnologies Inc, Columbia, Maryland 21046, USA.
Mult Scler. 1998 Dec;4(6):490-6. doi: 10.1177/135245859800400606.
We examined cerebral spinal fluid (CSF) from multiple sclerosis (MS) patients and patients with other neurological diseases (OND) for antibody specific for Human Herpesvirus-6 (HHV-6) and for HHV-6 DNA detectable by PCR. CSF from MS patients had a higher frequency of IgG antibody to HHV-6 late antigens (39.4%) compared with CSF from OND (7.4%). In contrast, the frequency of detectable IgG antibody in CSF from MS patients specific for Epstein-Barr Virus (EBV) (12.1%) and Human Cytomegalovirus (HCMV) (6.1%) was much lower. Two of 12 MS CSFs (16.7%) also contained HHV-6 DNA detected by PCR. None of four OND CSF were positive for HHV-6 DNA. Plasma from 16 patients with MS, eight with OND and 72 healthy donors were tested for antibodies by ELISA to HHV-6 early (p41/38) and late (gp110) proteins. Although no differences in anti-gp110 IgG antibody were detected between MS patients, patients with other neurological diseases, and normals, IgG antibody to early protein p41/38 was detected in > 68% of the plasma from MS patients, 12.5% from OND patients and 27.8% of the controls. IgM antibody to p41/38 was present in > 56% of MS patients, 12.5% of OND patients, and 19% of controls. These data suggest that more than half of the MS patients had active, ongoing HHV-6 infections. HHV-6 was also isolated from peripheral blood mononuclear cells (PBMC) from 3/5 MS patients who were in relapse or had progressive disease and was identified as HHV-6 Variant B. These preliminary results support the hypothesis that HHV-6 may be a co-factor in the pathogenesis of some cases of MS.
我们检测了来自多发性硬化症（MS）患者以及患有其他神经系统疾病（OND）患者的脑脊液（CSF），以寻找针对人类疱疹病毒6型（HHV - 6）的特异性抗体以及通过聚合酶链反应（PCR）可检测到的HHV - 6 DNA。与OND患者的脑脊液（7.4%）相比，MS患者的脑脊液中针对HHV - 6晚期抗原的IgG抗体频率更高（39.4%）。相比之下，MS患者脑脊液中针对爱泼斯坦 - 巴尔病毒（EBV）（12.1%）和人类巨细胞病毒（HCMV）（6.1%）的可检测到的IgG抗体频率要低得多。12份MS患者的脑脊液中有2份（16.7%）通过PCR检测也含有HHV - 6 DNA。4份OND患者的脑脊液中没有一份HHV - 6 DNA呈阳性。通过酶联免疫吸附测定（ELISA）检测了16例MS患者、8例OND患者和72名健康供者血浆中针对HHV - 6早期（p41/38）和晚期（gp110）蛋白的抗体。尽管在MS患者、其他神经系统疾病患者和正常人之间未检测到抗gp110 IgG抗体存在差异，但在超过68%的MS患者血浆、12.5%的OND患者血浆和27.8%的对照血浆中检测到了针对早期蛋白p41/38的IgG抗体。针对p41/38的IgM抗体存在于超过56%的MS患者、12.5%的OND患者和19%的对照中。这些数据表明，超过一半的MS患者存在活跃的、正在进行的HHV - 6感染。HHV - 6也从3/5处于复发期或患有进展性疾病的MS患者的外周血单个核细胞（PBMC）中分离出来，并被鉴定为HHV - 6 B型变异株。这些初步结果支持了HHV - 6可能是某些MS病例发病机制中的一个辅助因素这一假说。
