Liu Hai Yan, He Fang, Wang Jing Ping, Xi Ting Ye, Wang Hong Gang, Li Xing Feng
Subcentre of National Wheat Improvement Center, Agronomy College of Shandong Agricultural University, Taian 271018.
Fen Zi Xi Bao Sheng Wu Xue Bao. 2008 Apr;41(2):139-44.
A germplasm line Shannong05078 was developed from the progenies of Am3 x Triticum aestivum-Dasypyrum villosum amphidiploid, the purpose of this study is to ascertain the genome composition of Shannong05078 and then supply wheat breeding with reference. The results showed that Shannong05078 was stable in morphology characters and presenting favorable agronomic traits, it was resistant to powdery mildew and rust. Its protein content was higher, and the HWM-GS of it was 7+8, 5+10. The chromosome number of Shannong05078 was 2n=28, and the chromosome configuration was 2n=14 II at the PMC MI. SSR analysis showed that Shannong05078 contained the whole A and B genomes, and had little genetic materials from D genome. A specific primer of Dasypyrum villosum (phv29) was used to identify the genetic materials form Dasypyrum villosum in Shannong05078, and specific band of Dasypyrum villosum appeared in it. It can be deduced that the genomic composition of Shannong05078 is 2n=AABB=28, and genetic materials of D genome and Dasypyrum villosum were translated into Shannong05078.
种质系山农05078由Am3与普通小麦 - 绒毛草双二倍体的后代选育而成，本研究旨在确定山农05078的基因组组成，为小麦育种提供参考。结果表明，山农05078形态性状稳定，农艺性状优良，对白粉病和锈病具有抗性。其蛋白质含量较高，高分子量谷蛋白亚基组成为7 + 8、5 + 10。山农05078的染色体数为2n = 28，花粉母细胞减数分裂中期I染色体构型为2n = 14Ⅱ。SSR分析表明，山农05078含有完整的A和B基因组，几乎没有来自D基因组的遗传物质。利用绒毛草特异引物（phv29）对山农05078中来自绒毛草的遗传物质进行鉴定，在其中出现了绒毛草的特异条带。由此推断，山农05078的基因组组成为2n = AABB = 28，且含有D基因组和绒毛草的遗传物质。
