Deng Guang-Bing, Chen Jing, Ma Xin-Rong, Pan Zhi-Fen, Yu Mao-Qun, Li Xu-Feng
The College of Science, Sichuan University, Chengdu 610064, China.
Yi Chuan. 2004 Mar;26(2):189-94.
"Aegilops tauschii x Dasypyrum villosum" F1 hybrids were obtained by the combination of hybridization and embryo culture in vitro. Chromosome pairing behavior in meiosis of the hybrid F1 was carried out. Results showed that in an average , 1.25 rod bivalents were observed in one PMC, meiotic configuration was 2n=14=11.49 I + 1.25 II (Xta=1.25) and most of PMCs possessed 1 approximately 5(rod) bivalens, indicating that the relatively high homeology was detected between the D genome of Ae. tauschii and the V genome of D. villosum. The morphological differences between F1 hybrids and their parents were significant. F1 plants were highly self-sterile, but partially self-fertile after treated by chromosome doubling technique.
通过杂交和体外胚胎培养相结合的方法获得了“节节麦×绒毛草”F1杂种。对杂种F1减数分裂过程中的染色体配对行为进行了研究。结果表明,平均每个花粉母细胞观察到1.25个棒状二价体,减数分裂构型为2n = 14 = 11.49Ⅰ + 1.25Ⅱ(交叉端化值 = 1.25),大多数花粉母细胞具有1至5个(棒状)二价体,这表明在节节麦的D基因组和绒毛草的V基因组之间检测到了较高的同源性。F1杂种与其亲本之间的形态差异显著。F1植株高度自交不育,但经染色体加倍技术处理后部分可自交结实。