Donovan R M, Dickover R E, Goldstein E, Huth R G, Carlson J R
Department of Internal Medicine, University of California, Davis 95616.
J Acquir Immune Defic Syndr (1988). 1991;4(8):766-9.
The effect of treatment with 400-1,200 mg/day of zidovudine (ZDV) on HIV DNA concentrations in patient peripheral blood mononuclear cells (PBMCs) was studied in six patients during a 5- to 14-month period of therapy. HIV DNA was measured in PBMCs at intervals using a recently developed quantitative polymerase chain reaction assay. The amount of HIV DNA ranged from 2,000 to 40,000 copies of provirus per microgram of cellular DNA. The HIV provirus copy number showed little change with time in five patients, and increased and then remained constant in one patient. Thus, prolonged treatment with ZDV does not decrease the levels of HIV DNA in PBMCs.
在6名患者接受为期5至14个月的治疗期间，研究了每天服用400 - 1200毫克齐多夫定（ZDV）对患者外周血单个核细胞（PBMC）中HIV DNA浓度的影响。使用最近开发的定量聚合酶链反应测定法定期测量PBMC中的HIV DNA。HIV DNA的量为每微克细胞DNA中2000至40000个前病毒拷贝。5名患者的HIV前病毒拷贝数随时间变化不大，1名患者的拷贝数先增加然后保持稳定。因此，长期使用ZDV治疗不会降低PBMC中HIV DNA的水平。
