Hufert F T, von Laer D, Fenner T E, Schwander S, Kern P, Schmitz H
Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Federal Republic of Germany.
Arch Virol. 1991;120(3-4):233-40. doi: 10.1007/BF01310478.
We present data on the distribution of human immunodeficiency virus (HIV-1) proviral DNA in different subsets of peripheral blood mononuclear cells (PBMCs) over an observation period of eight months. Eleven patients with well documented HIV-1 infection were studied. The PBMCs were obtained at two intervals and purified by fluorescence-activated cell sorting (FACS) after staining with FITC-labelled monoclonal antibodies. Varying numbers of FACS-sorted CD4+ cells, CD8+ cells and peripheral monocytes were assayed for HIV-1 proviral DNA (env and gag region) by PCR. Samples from patients at CDC stages II or III had to contain 10(3)-10(4) cells in order to allow detection of proviral HIV-1 DNA. At CDC stage IV, however, HIV-1 DNA was detected in as few as 100 CD4+ T-lymphocytes. In contrast, in peripheral monocytes HIV-1 DNA was not regularly found. CD8+ cells did not harbor detectable amounts of proviral DNA. During an observation period of eight months, the rate of infected CD4+ T-lymphocytes increased significantly in three patients while staying constant in the remaining eight patients. This increase of the infection rate was paralleled by clinical progression in one patient and by a decrease of the absolute number of CD4+ cells in another patient. The percentage of CD4+ cells harboring the viral genome increases in the course of the disease. These results may help to explain the decrease in CD4+ T-lymphocyte counts during HIV-1 infection.
我们展示了在八个月的观察期内，人类免疫缺陷病毒（HIV-1）前病毒DNA在外周血单个核细胞（PBMC）不同亚群中的分布数据。研究了11例有充分记录的HIV-1感染患者。在两个时间点获取PBMC，并在用异硫氰酸荧光素（FITC）标记的单克隆抗体染色后，通过荧光激活细胞分选（FACS）进行纯化。通过聚合酶链反应（PCR）检测不同数量经FACS分选的CD4+细胞、CD8+细胞和外周单核细胞中的HIV-1前病毒DNA（env和gag区域）。来自疾病控制与预防中心（CDC）II期或III期患者的样本必须含有10³-10⁴个细胞，以便检测到前病毒HIV-1 DNA。然而，在CDC IV期，在低至100个CD4+ T淋巴细胞中就能检测到HIV-1 DNA。相比之下，在外周单核细胞中未经常发现HIV-1 DNA。CD8+细胞中未检测到可检测量的前病毒DNA。在八个月的观察期内，三名患者中受感染的CD4+ T淋巴细胞比例显著增加而其余八名患者保持不变。一名患者的感染率增加伴随着临床进展，另一名患者的CD4+细胞绝对数量减少。携带病毒基因组的CD4+细胞百分比在疾病过程中增加。这些结果可能有助于解释HIV-1感染期间CD4+ T淋巴细胞计数的下降。
