Yu Chang-You, Liu Yi-Wen, Chou Shih-Jen, Chao Maw-Rong, Weng Bor-Chun, Tsay Jwu-Guh, Chiu Cheng-Hsun, Ching Wu Ching, Long Lin Tsang, Chang Chih-Cheng, Chu Chishih
Department of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan.
Avian Pathol. 2008 Jun;37(3):273-9. doi: 10.1080/03079450802056546.
Riemerella anatipestifer causes infectious serositis of ducks and geese. The genomic diversity of R. anatipestifer associated with outbreaks in waterfowls was studied using 24 multidrug-resistant R. anatipestifer isolates collected from the visceral organs of ducks and geese from seven outbreaks in four goose farms and one outbreak in one duck farm. Seven methods were used to differentiate these isolates. Plasmid patterns differed in plasmid number and size, ranging from 2.9 kb to 20 kb, and provided seven profiles. Divergent nucleotide sequences (predominant in 670 to 830 base pairs) of the ompA gene categorized the 24 isolates into three groups based on cluster analysis and polymerase chain reaction-restriction fragment length polymorphism. Repetitive-sequence polymerase chain reaction and pulsed-field gel electrophoresis analysis revealed the highest genotypic variations among the isolates. Genotypes and serotypes differed among farms and within the same farm and even within a single goose. In conclusion, a difference in R. anatipestifer genotypes and serotypes was observed for multiple outbreaks in waterfowls.
鸭疫里默氏杆菌可引起鸭和鹅的传染性浆膜炎。利用从四个养鹅场的七次疫情以及一个养鸭场的一次疫情中采集的24株来自鸭和鹅内脏器官的多重耐药鸭疫里默氏杆菌分离株，研究了与水禽疫情相关的鸭疫里默氏杆菌的基因组多样性。使用七种方法对这些分离株进行鉴别。质粒图谱在质粒数量和大小上存在差异，范围从2.9 kb到20 kb，并提供了七种图谱。基于聚类分析和聚合酶链反应-限制性片段长度多态性，ompA基因的不同核苷酸序列（主要在670至830个碱基对之间）将24株分离株分为三组。重复序列聚合酶链反应和脉冲场凝胶电泳分析显示分离株之间存在最高的基因型变异。不同养殖场之间、同一养殖场内甚至同一只鹅体内的基因型和血清型都有所不同。总之，在水禽的多次疫情中观察到鸭疫里默氏杆菌基因型和血清型存在差异。
