Pathanasophon Pornpen, Phuektes Patchara, Tanticharoenyos Tipa, Narongsak Watcharachai, Sawada Takuo
National Institute of Animal Health, Kasetklang, Chatuchak, Bangkok 10900, Thailand.
Avian Pathol. 2002 Jun;31(3):267-70. doi: 10.1080/03079450220136576.
Eighty isolates of Riemerella anatipestifer representing 71 outbreaks of riemerellosis in Thailand between 1994 and 1999 were serotyped using the gel diffusion precipitin test. Based on the precipitation patterns, 25 serological profiles containing one to three antigenic determinants were recognized. Heat-stable antigens of the organism reacted with antisera raised against 16 known serotypes and an untypable strain 698/95. The most prevalent serotype appeared to be serotype 7, followed by serotypes 5, 10, 21 and 1. Further study demonstrated that the untypable strain probably represents a new serotype. Analysis of the polymerase chain reaction-amplified rrs genes for restriction fragment length polymorphisms verified the inclusion of strain 698/95 within the species R. anatipestifer and supported earlier work excluding strain 670/89, which had originally been designated the reference strain of serotype 20. Therefore, it is suggested that the strain 698/95 could be adopted as a replacement for the reference strain of serotype 20. Attention should be paid to strains with multiple antigenic factors as they may be useful for the preparation of vaccines.
利用凝胶扩散沉淀试验对1994年至1999年间泰国71起鸭疫里默氏菌病疫情中的80株鸭疫里默氏菌进行了血清分型。根据沉淀模式，识别出了25种包含一至三个抗原决定簇的血清学图谱。该菌的热稳定抗原与针对16种已知血清型和一株无法分型的菌株698/95制备的抗血清发生反应。最常见的血清型似乎是血清型7，其次是血清型5、10、21和1。进一步研究表明，该无法分型的菌株可能代表一种新的血清型。对聚合酶链反应扩增的rrs基因进行限制性片段长度多态性分析，证实菌株698/95属于鸭疫里默氏菌物种，并支持早期排除菌株670/89的工作，该菌株最初被指定为血清型20的参考菌株。因此，建议采用菌株698/95替代血清型20的参考菌株。应关注具有多种抗原因子的菌株，因为它们可能有助于疫苗的制备。
