von Byern J, Rudoll L, Cyran N, Klepal W
University of Vienna, Cell Imaging and Ultrastructure Research, Vienna, Austria.
Biotech Histochem. 2008 Feb;83(1):29-46. doi: 10.1080/10520290801999316.
An adhesive organ is a prominent characteristic of the genus Idiosepius. Histological, histochemical and ultrastructural methods were applied to elucidate the nature of secretion of the epithelial cells of three Idiosepius species. The adhesive organs of Idiosepius biserialis and Idiosepius pygmaeus consist of five distinct cell types that can be distinguished morphologically and by the composition of their secretions. Histochemical analysis revealed that three cell types contain different sugar units and basic proteins, whereas the interstitial cells lacked secretory material. Acidic and sulfated substances were absent in Idiosepius secretions. The adhesive organ, but not the secretory material of the glandular cells, contained O-linked oligosaccharides. The histochemical analysis of the secretory products suggested that adhesion and release are not effected by a "duo-gland" adhesive system as in Euprymna scolopes. Idiosepius presumably uses a transitory adhesion, perhaps induced by secretion of a highly viscous gel. Release might be caused by contraction of the mantle musculature and/or chemical release mechanisms such as dilutors or enzymes.
附着器是艾氏乌贼属的一个显著特征。运用组织学、组织化学和超微结构方法来阐明三种艾氏乌贼属物种上皮细胞的分泌性质。双列艾氏乌贼和侏儒艾氏乌贼的附着器由五种不同的细胞类型组成，这些细胞类型可通过形态学及其分泌物组成加以区分。组织化学分析显示，三种细胞类型含有不同的糖单元和碱性蛋白，而间质细胞缺乏分泌物质。艾氏乌贼的分泌物中不存在酸性和硫酸化物质。附着器含有O-连接寡糖，但腺细胞的分泌物质中没有。分泌产物的组织化学分析表明，与斯氏耳乌贼不同，艾氏乌贼的附着和释放并非由“双腺”附着系统实现。艾氏乌贼可能利用的是短暂附着，或许是由高粘性凝胶的分泌所诱导。释放可能是由外套膜肌肉组织的收缩和/或诸如扩张肌或酶等化学释放机制引起的。
