Dwyer B E, Nishimura R N, de Vellis J, Clegg K B
Molecular Neurobiology Laboratory, VA Medical Center, Sepulveda, California 91343.
J Neurosci Res. 1991 Mar;28(3):352-8. doi: 10.1002/jnr.490280306.
Rat forebrain astrocytes synthesize heat shock proteins with molecular weights 97, 89, 70, 68, and 30-34 kilodaltons. The stress inducible 68-kDa heat shock protein (HSP-68) was vigorously expressed by astrocytes in culture after a 45 degrees C, 20 min heat shock. HSP-68 synthesis was poorly inducible by a second heat shock given 16 hr after the initial heat shock. Decreased [35S]methionine incorporation into HSP-68 correlated with low levels of HSP-68 mRNA present after the second heat shock. The data suggest that control of HSP-68 mRNA levels by transcriptional/posttranscriptional mechanisms is a major site for regulation of HSP-68 synthesis.
大鼠前脑星形胶质细胞能合成分子量分别为97、89、70、68以及30 - 34千道尔顿的热休克蛋白。在45摄氏度热休克20分钟后，培养的星形胶质细胞会强烈表达应激诱导的68千道尔顿热休克蛋白（HSP - 68）。在首次热休克16小时后给予第二次热休克，HSP - 68的合成诱导效果较差。第二次热休克后，[35S]甲硫氨酸掺入HSP - 68的量减少，这与HSP - 68 mRNA水平较低相关。数据表明，通过转录/转录后机制控制HSP - 68 mRNA水平是调节HSP - 68合成的主要位点。
