Yin Dongguang, Cui Daxiang, Gao Feng, He Rong, He Youfeng, Liu Yibing, Shen Decun, Wu Minghong
Shanghai Applied Radiation Institute, College of Environmental and Chemical Engineering, Shanghai University, Jiadin, PR China.
J Immunoassay Immunochem. 2008;29(3):257-65. doi: 10.1080/15321810802119075.
A novel chemiluminescence immunoassay (CLIA) of total thyroxin (TT4) with the acridinium ester of DMAE x NHS [2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-10-methyl-acridinium-9-carboxylate methosulfate] labeled has been developed. In our method, microwells were coated with anti-T4 monoclonal antibody (McAb) and DMAE x NHS synthesized by our laboratory was conjugated with streptavidin (SA). T4-BSA was conjugated with biotin-N-hydroxysuccinimide (B x NHS), T4-BSA- B x NHS and the T4 in the standard or sample competitively react with anti-T4 McAb. Streptavidin-biotin separation and enhanced techniques were applied in this assay. The presented approach shows many excellent characteristics, particularly, rapid assay process and high detection sensitivity.
一种采用[2',6'-二甲基-4'-(N-琥珀酰亚胺氧基羰基)苯基-10-甲基-吖啶鎓-9-羧酸甲酯硫酸甲酯]标记的吖啶鎓酯的新型总甲状腺素（TT4）化学发光免疫分析（CLIA）方法已被开发出来。在我们的方法中，微孔板用抗T4单克隆抗体（McAb）包被，我们实验室合成的DMAE x NHS与链霉亲和素（SA）偶联。T4-BSA与生物素-N-羟基琥珀酰亚胺（B x NHS）偶联，T4-BSA-B x NHS与标准品或样品中的T4与抗T4 McAb竞争性反应。该分析采用了链霉亲和素-生物素分离和增强技术。所提出的方法显示出许多优异的特性，特别是分析过程快速且检测灵敏度高。
