Saxena Ashish, Nath Prasad Kashi, Chand Ghoshal Uday, Krishnani Narendra, Roshan Bhagat Monty, Husain Nuzhat
Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
Scand J Gastroenterol. 2008;43(6):669-74. doi: 10.1080/00365520801909660.
Helicobacter pylori and Epstein-Barr virus (EBV) infections are common world-wide. Though H. pylori infection is a major factor in gastroduodenal diseases, its role in association with EBV infection is unknown. We prospectively studied the association of H. pylori and EBV in patients with gastric cancer (GC) and peptic ulcer disease (PUD).
A total of 348 adult patients (non-ulcer dyspepsia (NUD) 241, PUD 45, GC 62) undergoing upper gastrointestinal endoscopy between September 2003 and May 2007 were enrolled in the study. H. pylori infection was diagnosed by rapid urease test, culture, histopathology and polymerase chain reaction (PCR). EBV DNA was detected by non-polymorphic Epstein-Barr nuclear antigen-1 (EBNA-1) gene-based PCR and sequence analysis.
The rate of H. pylori infection was higher in patients with PUD than in those with GC (80% versus 56.5%, p=0.01) and NUD (80% versus 55.2%, p=0.002). In patients with GC and PUD, EBV DNA was detected more often than in those with NUD (GC versus NUD - 82.3% versus 37.3%, p<0.001; PUD versus NUD - 75.5% versus 37.3%, p<0.001). H. pylori infection and EBV DNA detected in different groups of patients was as follows: 62.2% in PUD, 46.8% in GC and 29.5% in NUD. PUD and GC were significantly associated (p<0.001 and <0.05, respectively) with the presence of H. pylori infection and EBV DNA as compared with NUD.
EBV DNA either alone or in combination with H. pylori infection was significantly associated with GC and PUD, suggesting that EBV might play an important role in gastroduodenal pathology.
幽门螺杆菌和爱泼斯坦-巴尔病毒（EBV）感染在全球范围内都很常见。虽然幽门螺杆菌感染是胃十二指肠疾病的主要因素，但其与EBV感染相关的作用尚不清楚。我们前瞻性地研究了胃癌（GC）和消化性溃疡病（PUD）患者中幽门螺杆菌与EBV的关联。
2003年9月至2007年5月期间接受上消化道内镜检查的348例成年患者（非溃疡性消化不良（NUD）241例、PUD 45例、GC 62例）纳入本研究。幽门螺杆菌感染通过快速尿素酶试验、培养、组织病理学和聚合酶链反应（PCR）诊断。EBV DNA通过基于非多态性爱泼斯坦-巴尔核抗原-1（EBNA-1）基因的PCR和序列分析进行检测。
PUD患者的幽门螺杆菌感染率高于GC患者（80%对56.5%，p=0.01）和NUD患者（80%对55.2%，p=0.002）。在GC和PUD患者中，EBV DNA的检测频率高于NUD患者（GC对NUD - 82.3%对37.3%，p<0.001；PUD对NUD - 75.5%对37.3%，p<0.001）。不同组患者中检测到的幽门螺杆菌感染和EBV DNA情况如下：PUD组为62.2%，GC组为46.8%，NUD组为29.5%。与NUD相比，PUD和GC分别与幽门螺杆菌感染和EBV DNA的存在显著相关（p<0.001和<0.05）。
EBV DNA单独或与幽门螺杆菌感染联合与GC和PUD显著相关，提示EBV可能在胃十二指肠病理学中起重要作用。
