Nogueira Célia, Mota Marta, Gradiz Rui, Cipriano Maria Augusta, Caramelo Francisco, Cruz Hugo, Alarcão Ana, E Sousa Francisco Castro, Oliveira Fernando, Martinho Fernando, Pereira João Moura, Figueiredo Paulo, Leitão Maximino
Microbiology, Faculty of Medicine of the University of Coimbra, 3004-504 Coimbra, Portugal.
CIMAGO, Faculty of Medicine of the University of Coimbra, 3001-301 Coimbra, Portugal.
Infect Agent Cancer. 2017 Jul 19;12:41. doi: 10.1186/s13027-017-0151-8. eCollection 2017.
Gastric cancer (GC) is one of the most common malignant tumors of the digestive tract and is the third leading cause of cancer death worldwide. Epstein-Barr virus (EBV) has been associated with approximately 10% of the total cases of gastric carcinomas. No previous study has analyzed the prevalence of EBV infection in gastric cancer of the Portuguese population.
In the present study, we have analyzed 82 gastric carcinoma cases and 33 healthy individuals (control group) from Coimbra region for the presence of EBV by polymerase chain reaction (PCR) and by in situ hybridization (ISH) for EBV-encoded small RNAs (EBERs). The status of infection was assessed by serology and by PCR.
EBV was detected by PCR in 90.2% of stomach cancer cases, whereas EBERs were detected in 11%. In our series, EBV-associated gastric carcinoma (EBVaGC) were significantly associated with gender and the majority of them presented lymph node metastasis. These cases were generally graded in more advanced pTNM stages and, non-surprisingly, showed worse survival. infection was detected in 62.2% of the gastric cancers and 64.7% of these patients were CagA+. On the other hand, the prevalence was higher in the EBV-negative gastric carcinomas (64.4%) than in those carcinoma cases with EBV+ (44.4%).
The present study shows that prevalence of EBVaGC among Portuguese population is in accordance with the worldwide prevalence. EBV infection seems to be associated to poorer prognostic and no relation to infection has been found. Conversely, the presence of seems to have a favourable impact on patient's survival. Our results emphasize that geographic variation can contribute with new epidemiological data on the association of EBV with gastric cancer.
胃癌是最常见的消化道恶性肿瘤之一，是全球癌症死亡的第三大主要原因。爱泼斯坦-巴尔病毒（EBV）与约10%的胃癌病例相关。此前尚无研究分析葡萄牙人群胃癌中EBV感染的患病率。
在本研究中，我们通过聚合酶链反应（PCR）和针对EBV编码小RNA（EBERs）的原位杂交（ISH），分析了来自科英布拉地区的82例胃癌病例和33名健康个体（对照组）中EBV的存在情况。通过血清学和PCR评估感染状态。
通过PCR在90.2%的胃癌病例中检测到EBV，而通过ISH在11%的病例中检测到EBERs。在我们的系列研究中，EBV相关胃癌（EBVaGC）与性别显著相关，且大多数病例出现淋巴结转移。这些病例通常处于更晚期的pTNM分期，不出所料，其生存率较差。在62.2%的胃癌中检测到感染，其中64.7%的患者CagA呈阳性。另一方面，EBV阴性胃癌中的感染患病率（64.4%）高于EBV阳性的胃癌病例（44.4%）。
本研究表明，葡萄牙人群中EBVaGC的患病率与全球患病率一致。EBV感染似乎与较差的预后相关，且未发现与感染有相关性。相反，感染的存在似乎对患者的生存有有利影响。我们的结果强调，地理差异可为EBV与胃癌关联的新流行病学数据提供依据。
