马动脉炎病毒通过网格蛋白依赖的内吞作用被递送至宿主细胞的酸性区室。
Equine arteritis virus is delivered to an acidic compartment of host cells via clathrin-dependent endocytosis.
作者信息
Nitschke Matthias, Korte Thomas, Tielesch Claudia, Ter-Avetisyan Gohar, Tünnemann Gisela, Cardoso M Cristina, Veit Michael, Herrmann Andreas
机构信息
Department of Biology/Biophysics, Humboldt University Berlin, Invalidenstr. 42, 10115 Berlin, Germany.
出版信息
Virology. 2008 Aug 1;377(2):248-54. doi: 10.1016/j.virol.2008.04.041.
Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae. Infection by EAV requires the release of the viral genome by fusion with the respective target membrane of the host cell. We have investigated the entry pathway of EAV into Baby Hamster Kidney cells (BHK). Infection of cells assessed by the plaque reduction assay was strongly inhibited by substances which interfere with clathrin-dependent endocytosis and by lysosomotropic compounds. Furthermore, infection of BHK cells was suppressed when clathrin-dependent endocytosis was inhibited by expression of antisense RNA of the clathrin-heavy chain before infection. These results strongly suggest that EAV is taken up via clathrin-dependent endocytosis and is delivered to acidic endosomal compartments.
马动脉炎病毒（EAV）是一种有包膜的正链RNA病毒，属于动脉炎病毒科。EAV感染需要通过与宿主细胞的相应靶膜融合来释放病毒基因组。我们研究了EAV进入幼仓鼠肾细胞（BHK）的途径。通过蚀斑减少试验评估的细胞感染受到干扰网格蛋白依赖性内吞作用的物质和溶酶体促渗化合物的强烈抑制。此外，在感染前通过表达网格蛋白重链的反义RNA抑制网格蛋白依赖性内吞作用时，BHK细胞的感染也受到抑制。这些结果强烈表明，EAV是通过网格蛋白依赖性内吞作用被摄取，并被递送至酸性内体区室。
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