Zhou Zhijun, Shi Fuming, Huang Yuan
College of Life Science, Shaanxi Normal University, Xi'an, China.
J Genet Genomics. 2008 Jun;35(6):341-8. doi: 10.1016/S1673-8527(08)60050-8.
The complete mitochondrial genome (mitogenome) of Gampsocleis gratiosa was determined. The 15,929 bp in the size of G. gratiosa mitogenome contains a typical gene content, base composition, and codon usage found in metazoan. All 13 protein coding genes (PCGs) of the G. gratiosa mitogenome start with a typical ATN codon. The usual termination codons (TAA and TAG) were found from 10 PCGs. However, the atp6, nad4, and nad5 had incomplete termination codon (T). The anticodons of all tRNAs are identical to those observed in Drosophila yakuba and Locusta migratoria, and can be folded in the form of a typical clover leaf structure except for trnS (AGN). The secondary structure of trnS (AGN) was drawn according with the Steinberg-Cedergren tertiary structure. The A+T content (67.4%) of the A+T-rich region is relatively lower among the mitogenome regions, in contrast, it usually contains the highest A+T content for most insects. Two isolated sequence repeat regions (202 bp) were found in the A+T-rich region with mapping and secondary structure information.
测定了优雅蝈螽的完整线粒体基因组（线粒体基因组）。优雅蝈螽线粒体基因组大小为15,929 bp，包含后生动物中典型的基因组成、碱基组成和密码子使用情况。优雅蝈螽线粒体基因组的所有13个蛋白质编码基因（PCGs）均以典型的ATN密码子起始。在10个PCGs中发现了常见的终止密码子（TAA和TAG）。然而，atp6、nad4和nad5具有不完整的终止密码子（T）。所有tRNA的反密码子与在雅库布果蝇和飞蝗中观察到的相同，除了trnS（AGN）外，均可折叠成典型的三叶草叶结构。trnS（AGN）的二级结构是根据斯坦伯格 - 塞德格伦三级结构绘制的。富含A + T区域的A + T含量（67.4%）在线粒体基因组区域中相对较低，相比之下，大多数昆虫的该区域通常含有最高的A + T含量。在富含A + T区域中发现了两个分离的序列重复区域（202 bp），并带有定位和二级结构信息。
