Miyazaki Yoshiyuki, Shimanoe Yohei, Wang Seng, Yoshida Hiroki
Department of Biomolecular Sciences, Division of Molecular and Cellular Immunoscience, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan.
Biochem Biophys Res Commun. 2008 Aug 29;373(3):397-402. doi: 10.1016/j.bbrc.2008.06.038. Epub 2008 Jun 20.
Interleukin (IL-) 27 is a member of the IL-12 cytokine family. Although recent analyses of WSX-1 (IL-27 receptor alpha chain)-deficient mice as well as in vitro studies using recombinant IL-27 revealed the immunosuppressive function of IL-27, in vivo role and therapeutic potential of IL-27 remain poorly elucidated. Here we investigated the effect of IL-27 administration on delayed-type hypersensitivity (DTH). While WSX-1-deficient mice showed higher DTH responses as shown by the degree of footpad swelling, administration of IL-27 significantly ameliorated the footpad swelling. Since the activation status of the draining lymph node cells were not affected by IL-27 deficiency, it was suggested that IL-27 affected the effector phase of the response. These results collectively indicate that IL-27 has a suppressive effect on activated T cells in the experimental model and also has a therapeutic potential for some diseases caused by immune disorder.
白细胞介素（IL-）27是IL-12细胞因子家族的成员。尽管最近对WSX-1（IL-27受体α链）缺陷小鼠的分析以及使用重组IL-27的体外研究揭示了IL-27的免疫抑制功能，但IL-27在体内的作用和治疗潜力仍未得到充分阐明。在此，我们研究了给予IL-27对迟发型超敏反应（DTH）的影响。虽然WSX-1缺陷小鼠表现出更高的DTH反应，如足垫肿胀程度所示，但给予IL-27可显著改善足垫肿胀。由于引流淋巴结细胞的激活状态不受IL-27缺陷的影响，提示IL-27影响反应的效应阶段。这些结果共同表明，IL-27在实验模型中对活化的T细胞具有抑制作用，并且对一些由免疫紊乱引起的疾病也具有治疗潜力。
