Department of General Surgery, Tenth People's Hospital, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai 200072, China.
Bosn J Basic Med Sci. 2013 May;13(2):114-8. doi: 10.17305/bjbms.2013.2391.
The objective of our work was to evaluate the effect of interferon-γ (IFN-γ) on cytokine expression in rat acute pancreatitis (AP). AP was introduced to rats which were divided into Control, AP and IFN-γ group. Rats in the AP and IFN-γ group were sacrificed as 6, 12 and 24 h after IFN-γ treatment. The serum amylase (AMA), endotoxin and cytokines were detected. The pathological examination and immunofluorescence staining of pancreas for TNF-α, NF-κB and IL-18 were performed. The serum AMA increased significantly at 6 h and reduced at 48 h after AP. The increase in IFN-γ was higher than that in AMA. IL-18 increased in the AP and IFN group, and IFN increased markedly at 48 h after AP. IL-27 reduced at 24 h after AP compared with AP group. In the AP group, the immunostaining of cytokines increased. In the IFN group, the edema in the pancreas was more severe, and NF-κB and IL-18 expression was higher than that in the other two groups. IFN-γ can increase serum IL-18 and reduce IL-27 in AP. IFN-γ can increase serum IL-18 and reduce serum IL-27 in AP. The increase in NF-κB and IL-18 may exert influence on pro-inflammatory cytokines to deteriorate inflammation in the pancreas. Thus, to control the IFN-γ might has promise to attenuate pancreatitis.
我们的工作目的是评估干扰素-γ（IFN-γ）对大鼠急性胰腺炎（AP）细胞因子表达的影响。AP 被引入大鼠，大鼠被分为对照组、AP 组和 IFN-γ 组。AP 组和 IFN-γ 组大鼠分别在 IFN-γ 处理后 6、12 和 24 小时处死。检测血清淀粉酶（AMA）、内毒素和细胞因子。进行胰腺 TNF-α、NF-κB 和 IL-18 的病理检查和免疫荧光染色。AP 后 6 小时血清 AMA 明显升高，48 小时降低。IFN-γ 的增加高于 AMA。AP 和 IFN 组的 IL-18 增加，AP 后 48 小时 IFN 明显增加。与 AP 组相比，AP 后 24 小时 IL-27 减少。AP 组细胞因子免疫染色增加。IFN 组胰腺水肿更严重，NF-κB 和 IL-18 表达高于其他两组。IFN-γ 可增加 AP 血清中的 IL-18，降低 IL-27。IFN-γ 可增加 AP 血清中的 IL-18，降低 IL-27。NF-κB 和 IL-18 的增加可能会影响促炎细胞因子，从而加重胰腺炎症。因此，控制 IFN-γ 可能有助于减轻胰腺炎。
