多发性硬化症患者外周血单核细胞和脑脊液中肺炎衣原体DNA及mRNA转录水平

Chlamydophila pneumoniae DNA and mRNA transcript levels in peripheral blood mononuclear cells and cerebrospinal fluid of patients with multiple sclerosis.

作者信息

Contini Carlo, Seraceni Silva, Castellazzi Massimiliano, Granieri Enrico, Fainardi Enrico

机构信息

Section of Infectious Diseases, Department of Clinical and Experimental Medicine, University of Ferrara, Ferrara, Italy.

出版信息

Neurosci Res. 2008 Sep;62(1):58-61. doi: 10.1016/j.neures.2008.05.003. Epub 2008 May 20.

DOI:10.1016/j.neures.2008.05.003

PMID:18572268

Abstract

Chlamydophila pneumoniae DNA and mRNA transcripts were investigated by PCR and RT-PCR in fresh CSF and PBMC specimens co-cultured in Hep-2 cell lines and collected from 14 patients with definite RR MS and 19 patients with other inflammatory (OIND) and non-inflammatory (NIND) neurological controls. A positivity for C. pneumoniae DNA and mRNA was detected in CSF and PBMCs of 9 RR MS patients (64.2%) with evidence of disease activity, whereas only 3 controls were positive for Chlamydial DNA. These preliminary findings suggest that C. pneumoniae may occur in a persistent and metabolically active state at both peripheral and intrathecal levels in MS, but not in OIND and NIND.

摘要

采用聚合酶链反应（PCR）和逆转录聚合酶链反应（RT-PCR）技术，对14例确诊复发缓解型多发性硬化（RR MS）患者以及19例其他炎症性（OIND）和非炎症性（NIND）神经疾病对照患者的新鲜脑脊液（CSF）和外周血单核细胞（PBMC）标本进行检测，这些标本在人喉表皮样癌细胞系（Hep-2）中共培养。结果发现，9例有疾病活动证据的RR MS患者的脑脊液和外周血单核细胞中肺炎衣原体DNA和信使核糖核酸（mRNA）呈阳性（64.2%），而仅3例对照的衣原体DNA呈阳性。这些初步研究结果表明，肺炎衣原体可能以持续且代谢活跃的状态存在于MS患者的外周和鞘内，但在OIND和NIND患者中不存在。