Bechkoff Géraldine, Radisson Jacqueline, Bessueille Laurence, Bouchekioua-Bouzaghou Katia, Buchet René
Université de Lyon, Villeurbanne F-69622, France.
Biochem Biophys Res Commun. 2008 Aug 29;373(3):378-81. doi: 10.1016/j.bbrc.2008.06.044. Epub 2008 Jun 23.
Matrix vesicles (MVs) are involved in the initial step of mineralization in skeletal tissues and provide an easily model to analyze the hydroxyapatite (HA) formation. Sr stimulates bone formation and its effect was tested on MVs. Sr(2+) (15-50 microM) in the mineralization medium containing MVs, 2 mM Ca(2+) and 3.42 mM P(i), retarded HA formation. Sr(2+) (1-5 mM) in the same medium-induced other types of mineral than HA and cancelled the ATP-, ADP- or PP(i)-induced retardation in the mineral formation. Our findings suggest that the beneficial effect of Sr(2+) at a low dose (15-50 microM) is rather an inhibitor of bone resorption than an activator of mineral formation, while at high Sr(2+) concentration (1-5 mM), mineral formation, especially other types of mineral than HA, is favored.
基质小泡（MVs）参与骨骼组织矿化的初始步骤，并为分析羟基磷灰石（HA）形成提供了一个简便的模型。锶可刺激骨形成，并在MVs上测试了其效果。在含有MVs、2 mM钙离子和3.42 mM无机磷（Pi）的矿化培养基中，15 - 50 microM的锶离子（Sr(2+)）会延迟HA的形成。在相同培养基中，1 - 5 mM的Sr(2+)会诱导形成除HA之外的其他类型矿物质，并消除ATP、ADP或焦磷酸（PP(i)）诱导的矿物质形成延迟。我们的研究结果表明，低剂量（15 - 50 microM）的Sr(2+)的有益作用更像是骨吸收的抑制剂而非矿物质形成的激活剂，而在高Sr(2+)浓度（1 - 5 mM）时，矿物质形成，尤其是除HA之外的其他类型矿物质，会受到促进。
