基于表面等离子体共振技术的核酸传感器用于毒物检测的自组装单层膜。

Self-assembled monolayer for toxicant detection using nucleic acid sensor based on surface plasmon resonance technique.

作者信息

Solanki Pratima R, Prabhakar Nirmal, Pandey M K, Malhotra B D

机构信息

Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr K. S. Krishnan Road, New Delhi, 110012, India.

出版信息

Biomed Microdevices. 2008 Oct;10(5):757-67. doi: 10.1007/s10544-008-9188-1.

DOI:10.1007/s10544-008-9188-1

PMID:18574694

Abstract

Double stranded calf thymus deoxyribonucleic acid (dsCT-DNA) has been covalently immobilized onto self-assembled monolayer (SAM) of beta-merceptoethanol (MCE) on gold substrates via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry for fabrication of the surface plasmon resonance (SPR) based biosensing device. The dsCT-DNA-MCE-SAM/Au bioelectrode has been characterized using electrochemical impedance spectroscopy, cyclic voltammetry, contact angle measurements and atomic force microscopy, respectively. This biosensing device has been utilized for detection of cypermethrin (0.0005 ppm) using electrochemical and SPR techniques, respectively. The mechanism of interaction of cypermethrin with dsCT-DNA immobilized onto MCE-SAM has been proposed.

摘要

通过N-乙基-N'-(3-二甲氨基丙基)碳二亚胺和N-羟基琥珀酰亚胺化学方法，将双链小牛胸腺脱氧核糖核酸(dsCT-DNA)共价固定在金基底上的β-巯基乙醇(MCE)自组装单分子层(SAM)上，用于制备基于表面等离子体共振(SPR)的生物传感装置。分别采用电化学阻抗谱、循环伏安法、接触角测量和原子力显微镜对dsCT-DNA-MCE-SAM/Au生物电极进行了表征。该生物传感装置已分别用于采用电化学和SPR技术检测氯氰菊酯(0.0005 ppm)。提出了氯氰菊酯与固定在MCE-SAM上的dsCT-DNA的相互作用机制。