Solanki Pratima R, Prabhakar Nirmal, Pandey M K, Malhotra B D
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr K. S. Krishnan Road, New Delhi-110012, India.
J Mol Recognit. 2008 Jul-Aug;21(4):217-23. doi: 10.1002/jmr.888.
Nucleic acid sensor based on polyaniline (PANI) has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (ClO(-) (4))-doped PANI film deposited onto indium-tin-oxide (ITO) glass plate using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) chemistry. These dsCT-DNA-PANI-ClO(4)/ITO and PANI-ClO(4)/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, scanning electron microscopy (SEM) and Fourier-transform-infrared (FTIR) measurements. This disposable dsCT-DNA-PANI-ClO(4)/ITO bioelectrode, stable for about 4 months, can be used to detect cypermethrin (0.005 ppm) and trichlorfon (0.01 ppm) in 30 and 60 s, respectively.
基于聚苯胺（PANI）的核酸传感器是通过使用1-（3-二甲基氨基丙基）-3-乙基碳二亚胺盐酸盐（EDC）/N-羟基琥珀酰亚胺（NHS）化学方法，将双链小牛胸腺（dsCT）DNA共价固定在沉积于氧化铟锡（ITO）玻璃板上的高氯酸盐（ClO₄⁻）掺杂的聚苯胺薄膜上制备而成。这些dsCT-DNA-PANI-ClO₄/ITO和PANI-ClO₄/ITO电极已通过方波伏安法、电化学阻抗、扫描电子显微镜（SEM）和傅里叶变换红外（FTIR）测量进行了表征。这种一次性使用的dsCT-DNA-PANI-ClO₄/ITO生物电极可稳定约4个月，分别可在30秒和60秒内用于检测氯氰菊酯（0.005 ppm）和敌百虫（0.01 ppm）。
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