巴西圣保罗州绵羊弓形虫的血清阳性率及分离情况
Seroprevalence and isolation of Toxoplasma gondii from sheep from São Paulo state, Brazil.
作者信息
Ragozo A M A, Yai Ragozo L E O, Oliveira L N, Dias R A, Dubey J P, Gennari S M
机构信息
Department of Preventive Veterinary Medicine and Animal Health, Faculdade de Madicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
出版信息
J Parasitol. 2008 Dec;94(6):1259-63. doi: 10.1645/GE-1641.1.
Sheep are important in the epidemiology of Toxoplasma gondii infection but little is known of ovine toxoplasmosis in Brazil. Antibodies to T. gondii were assayed in sera of 495 sheep from 36 counties of São Paulo State, Brazil, using the modified agglutination test (MAT titer > or =1:25) and found in 120 (24.2%). Samples of brain, heart, and diaphragm of 82 seropositive sheep were pooled, digested in pepsin, and bioassayed in mice. Toxoplasma gondii was isolated from tissue homogenates of 16 sheep and the isolates were designated TgShBr1-16. Six of the 16 T. gondii isolates killed 100% of infected mice. Results indicate that asymptomatic sheep can harbor mouse-virulent T. gondii, and hence they can serve as a source of infection for humans.
绵羊在弓形虫感染的流行病学中具有重要意义，但巴西对绵羊弓形虫病的了解甚少。采用改良凝集试验（MAT滴度≥1:25）对来自巴西圣保罗州36个县的495只绵羊血清中的弓形虫抗体进行检测，发现120只（24.2%）呈阳性。将82只血清阳性绵羊的脑、心脏和膈肌样本混合，用胃蛋白酶消化后在小鼠身上进行生物测定。从16只绵羊的组织匀浆中分离出弓形虫，分离株命名为TgShBr1 - 16。16株弓形虫分离株中有6株导致100%的感染小鼠死亡。结果表明，无症状绵羊可携带对小鼠具有毒力的弓形虫，因此它们可作为人类的感染源。
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