Maru Moges, Damtie Debasu, Kenubih Ambaye, Maru Abiy, Adugna Biyansa, Dagnachew Shimelis, Tarekegn Zewdu Seyoum
Department of Veterinary Paraclinical Studies, College of Veterinary Medicine and Animal Sciences, University of Gondar, P.O. Box: 196, Gondar, Ethiopia.
Department of Immunology and Molecular Biology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, P.O. Box: 196, Gondar, Ethiopia.
J Parasit Dis. 2022 Jun;46(2):429-439. doi: 10.1007/s12639-022-01466-6. Epub 2022 Jan 22.
This study investigated the occurrence, isolation and virulence of in slaughtered domestic ruminants in Gondar, Northwest Ethiopia. Three hundred thirty-five blood samples (135 sheep, 50 goats, and 150 cattle) were purposefully collected from abattoirs and slaughterhouses. antibodies were assessed using a commercial Toxo-Latex agglutination test. Tissue digestion with the pepsin enzyme was also performed on 39 heart muscles of seropositive animals, and viable was isolated in white albino mice. As a result, the occurrence of infection was 55.8%. antibodies were found in 59.3% of cattle, 58% of goats, and 51.1% of sheep. The prevalence of antibodies in sheep was significantly higher in females (χ2 = 4.55, = 0.033) and adults (χ2 = 7.57, p = 0.006). Similarly, in cattle, the presence of antibodies was associated with old groups (χ2 = 7.81, = 0.005) and cross-breeds (χ2 = 6.30, = 0.012). The overall viable isolates in bioassayed mice were 38.5%, and the parasites were isolated from sheep (8/16), cattle (3/14) and goats (4/9) samples, with the majority of these isolates (87.2%) being avirulent. In conclusion, the presence of antibodies and a high proportion of viable in this study may indicate the parasite's prevalence and zoonotic importance in the study area. To plan control strategies, more research on the genotype and transmission dynamics of this parasite is required. Public education about transmission routes and control methods is critical for preventing transmission.
本研究调查了埃塞俄比亚西北部贡德尔屠宰的家养反刍动物中[寄生虫名称未给出]的发生、分离情况及毒力。从屠宰场和肉类加工厂有目的地采集了335份血样（135份绵羊血样、50份山羊血样和150份牛血样）。使用商业弓形虫乳胶凝集试验评估[寄生虫名称未给出]抗体。还对39份血清阳性动物的心肌组织进行了胃蛋白酶消化处理，并在白色白化小鼠中分离出活的[寄生虫名称未给出]。结果显示，[寄生虫名称未给出]感染的发生率为55.8%。在59.3%的牛、58%的山羊和51.1%的绵羊中发现了[寄生虫名称未给出]抗体。绵羊中[寄生虫名称未给出]抗体的流行率在雌性（χ2 = 4.55，p = 0.033）和成年羊（χ2 = 7.57，p = 0.006）中显著更高。同样，在牛中，[寄生虫名称未给出]抗体的存在与老龄组（χ2 = 7.81，p = 0.005）和杂交品种（χ2 = 6.30，p = 0.012）有关。在生物测定小鼠中分离出的活的[寄生虫名称未给出]总体分离率为38.5%，寄生虫从绵羊（8/16）、牛（3/14）和山羊（4/9）样本中分离得到，这些分离株中的大多数（87.2%）无毒力。总之，本研究中[寄生虫名称未给出]抗体的存在以及高比例的活的[寄生虫名称未给出]可能表明该寄生虫在研究区域的流行情况及人畜共患病重要性。为制定控制策略，需要对该寄生虫的基因型和传播动态进行更多研究。关于[寄生虫名称未给出]传播途径和控制方法的公众教育对于预防[寄生虫名称未给出]传播至关重要。
