印度南部钦奈市小型反刍动物（绵羊和山羊）的分离与特性分析
Isolation and characterization of from small ruminants (sheep and goats) in Chennai City, South India.
作者信息
Satbige Ajay Suryakant, Sreekumar C, Rajendran C, Vijaya Bharathi M
机构信息
Department of Preventive Medicine, Madras Veterinary College, Vepery, Chennai, Tamilnadu India.
Post Graduate Research Institute in Animal Sciences, Tamil nadu Veterinary and Animal Science University, Kattupakkam, Tamilnadu India.
出版信息
J Parasit Dis. 2017 Sep;41(3):869-873. doi: 10.1007/s12639-017-0908-4. Epub 2017 Mar 29.
The present study aimed for the isolation and genotyping of from small ruminants (sheep and goats). 14 out of 193 tissue samples (either brain and heart) tested positive by MDAT for anti- antibodies, were selected and bioassayed, which resulted 4 samples positive for after 40 days of post inoculation. Four samples consisting of 3 numbers of sheep and 1 number of goat tissues out of 14 samples detected by B1 PCR, were genotyped at SAG3 locus by nested polymerase chain reaction-restriction fragment length polymorphism technique (nPCR-RFLP). The results of the present study revealed that the four isolates designated as TgShIn19, TgShIn76, TgShIn77 and TgGtIn27 were circulating in small ruminants, were belonged to genotypes of type II (TgShIn19) and type III (TgShIn76, TgShIn77 and TgGtIn27) which are in concordance with the previously reported genotypes from other animal species and further this presumptive results indicating that the genotype II and III could be the predominant in different animal species including birds and humans in India.
本研究旨在从小反刍动物（绵羊和山羊）中分离并进行基因分型。193份组织样本（脑和心脏）中有14份经MDAT检测抗抗体呈阳性，被选取并进行生物测定，接种后40天有4份样本对[具体病原体名称未给出]呈阳性。通过B1 PCR检测出的14份样本中，由3份绵羊组织和1份山羊组织组成的4份样本，采用巢式聚合酶链反应-限制性片段长度多态性技术（nPCR-RFLP）在SAG3位点进行基因分型。本研究结果显示，命名为TgShIn19、TgShIn76、TgShIn77和TgGtIn27的4株分离株在小反刍动物中传播，属于II型（TgShIn19）和III型（TgShIn76、TgShIn77和TgGtIn27）基因型，这与先前报道的其他动物物种的基因型一致，进一步的推测结果表明，II型和III型基因型可能在包括印度鸟类和人类在内的不同动物物种中占主导地位。
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