一种用于监测霍乱弧菌O1生物型埃尔托新兴变体传播的错配扩增突变PCR检测方法的开发与验证
Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor.
作者信息
Morita Masatomo, Ohnishi Makoto, Arakawa Eiji, Bhuiyan N A, Nusrin Suraia, Alam Munirul, Siddique A K, Qadri Firdausi, Izumiya Hidemasa, Nair G Balakrish, Watanabe Haruo
机构信息
National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo, 162-8640, Japan.
出版信息
Microbiol Immunol. 2008 Jun;52(6):314-7. doi: 10.1111/j.1348-0421.2008.00041.x.
Abstract
A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.
摘要
开发并验证了一种错配扩增突变PCR检测方法，用于快速检测霍乱弧菌O1生物型特异性霍乱毒素B亚单位。该检测方法将有助于轻松监测霍乱弧菌O1埃尔托生物型新出现变体的传播情况。
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