Kaehr Bryan, Shear Jason B
Department of Chemistry and Biochemistry and the Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA.
Proc Natl Acad Sci U S A. 2008 Jul 1;105(26):8850-4. doi: 10.1073/pnas.0709571105. Epub 2008 Jun 25.
We report a method for creating stimuli-responsive biomaterials in which scanning nonlinear excitation is used to photocrosslink proteins at submicrometer 3D coordinates. Proteins with differing hydration properties can be combined to achieve tunable volume changes that are rapid and reversible in response to changes in chemical environment. Protein matrices having arbitrary 3D topographies and definable density gradients over micrometer dimensions provide the ability to effect rapid (<1 sec) and precise mechanical manipulations by means of changes in hydrogel size and shape, and applicability of these materials to cell biology is shown through the fabrication of responsive bacterial cages.
我们报告了一种创建刺激响应性生物材料的方法,其中扫描非线性激发用于在亚微米三维坐标处对蛋白质进行光交联。具有不同水化特性的蛋白质可以组合起来,以实现响应化学环境变化的快速且可逆的可调体积变化。具有任意三维形貌且在微米尺寸上具有可定义密度梯度的蛋白质基质,能够通过水凝胶大小和形状的变化实现快速(<1秒)且精确的机械操作,并且通过制造响应性细菌笼展示了这些材料在细胞生物学中的适用性。