飞秒激光双光子吸收特性诱导人角膜基质胶原交联的有效性。
Effectiveness of collagen cross-linking induced by two-photon absorption properties of a femtosecond laser in human corneal stroma.
作者信息
Chang Le, Zhang Lin, Cheng Zhenzhou, Zhang Nan, Wang Congzheng, Wang Yan, Liu Weiwei
机构信息
Clinical College of Ophthalmology, Tianjin Medical University, No. 22 Meteorological Terrace Road, Heping District, Tianjin 300070, China.
Tianjin Key Laboratory of Ophthalmology and Visual Science, Tianjin Eye Hospital, Tianjin Eye Institute, Nankai University Affiliated Eye Hospital, Nankai University, No. 4 Gansu Road, Heping District, Tianjin 300020, China.
出版信息
Biomed Opt Express. 2022 Sep 1;13(9):5067-5081. doi: 10.1364/BOE.468593.
This study aimed to investigate the effectiveness of two-photon induced collagen cross-linking (CXL) using femtosecond lasers in human corneal stroma. An 800-nm femtosecond laser optical path for CXL was established. Corneal samples that received two-photon induced CXL and ultraviolet-A (UVA) CXL underwent uniaxial stretching experiments, proteolytic resistance assays and observation of collagen fiber structure changes. Two-photon induced CXL can achieve corneal stiffening effects comparable to UVA CXL and showed better advantages at low strains. The cornea after two-photon induced CXL exhibited high enzymatic resistance and tight collagen fiber arrangement. Two-photon induced CXL promises to be a new option for keratoconus.
本研究旨在探讨飞秒激光双光子诱导角膜基质层胶原交联（CXL）的有效性。建立了用于CXL的800纳米飞秒激光光路。对接受双光子诱导CXL和紫外线A（UVA）CXL的角膜样本进行单轴拉伸实验、抗蛋白酶解测定以及胶原纤维结构变化观察。双光子诱导CXL可实现与UVA CXL相当的角膜硬化效果，且在低应变时显示出更好的优势。双光子诱导CXL后的角膜表现出高酶抗性和紧密的胶原纤维排列。双光子诱导CXL有望成为圆锥角膜治疗的新选择。
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