超快可逆光交联反应：迈向原位DNA操作
Ultrafast reversible photo-cross-linking reaction: toward in situ DNA manipulation.
作者信息
Yoshimura Yoshinaga, Fujimoto Kenzo
机构信息
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
出版信息
Org Lett. 2008 Aug 7;10(15):3227-30. doi: 10.1021/ol801112j. Epub 2008 Jun 27.
PMID:18582065
Abstract
We describe a novel ultrafast reversible DNA interstrand photo-cross-linking reaction via 3-cyanovinylcarbazole nucleoside ( (CNV)K). Oligodeoxynucleotide (ODN) containing (CNV)K can be photo-cross-linked by irradiation at 366 nm for 1 s, and the photo-cross-linked ODN can be split by irradiation at 312 nm for 60 s.
摘要
我们描述了一种通过3-氰基乙烯基咔唑核苷（(CNV)K）实现的新型超快可逆DNA链间光交联反应。含有(CNV)K的寡脱氧核苷酸（ODN）可通过在366 nm处照射1 s进行光交联，而光交联的ODN可通过在312 nm处照射60 s进行拆分。
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