光响应性人工核酸介导的链间DNA光交联

Interstrand DNA photocrosslinking by photoresponsive artificial nucleic acid.

作者信息

Yoshimura Yoshinaga, Ito Yoshiaki, Fujimoto Kenzo

机构信息

The School of Materials Science, JAIST, 1-1 Asahidai, Tatsunokuchi, Nomi, Ishikawa 923-1292, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2004(48):81-2. doi: 10.1093/nass/48.1.81.

DOI:10.1093/nass/48.1.81

PMID:17150488

Abstract

We report a novel interstrand photocrosslink of oligodeoxynucleotides (ODNs). In this system, a modified ODN containing 3-carbonylvinyl phenol nucleoside reacts by photoirradiation at 366 nm with adenine residue of a complementary template ODN to yield a crosslinked ODN.

摘要

我们报道了一种新型的寡脱氧核苷酸（ODN）链间光交联。在该体系中，含有3-羰基乙烯基苯酚核苷的修饰ODN通过366nm的光照射与互补模板ODN的腺嘌呤残基发生反应，生成交联的ODN。