3-氰基乙烯基咔唑核苷在 DNA 三螺旋体中的快速可逆光交联反应。
Quick and reversible photocrosslinking reaction of 3-cyanovinylcarbazole nucleoside in a DNA triplex.
机构信息
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahi-dai, Nomi, Ishikawa 923-1292, Japan.
出版信息
Org Biomol Chem. 2013 Aug 21;11(31):5065-8. doi: 10.1039/c3ob40915e. Epub 2013 Jun 13.
PMID:23764762
Abstract
3-Cyanovinylcarbazole nucleoside ((CNV)K), which can quickly photocrosslink to the pyrimidine base in the DNA double strand, was incorporated into a triplex forming oligonucleotide (TFO). We successfully demonstrated that (CNV)K in TFO can photocrosslink to the thymine base in Watson-Crick double-stranded DNA via anti-Hoogsteen base pairing with a few seconds of photoirradiation.
摘要
3-氰基乙烯基咔唑核苷((CNV)K)可以快速光交联到 DNA 双链中的嘧啶碱基，将其掺入三链体形成寡核苷酸(TFO)。我们成功地证明，TFO 中的(CNV)K 可以通过反 Hoogsteen 碱基配对与沃森-克里克双链 DNA 中的胸腺嘧啶碱基光交联，只需几秒钟的光照射。
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