Volf P, Grubhoffer L
Department of Parasitology and Hydrobiology, Faculty of Sciences, Charles University, Prague, Czechoslovakia.
Vet Parasitol. 1991 Mar;38(2-3):225-34. doi: 10.1016/0304-4017(91)90132-f.
The reaction of Polyplax spinulosa protein components with antigen-specific rat antisera and with five plant lectins is described. In immunoblotting, the antisera from rats infested with the louse P. spinulosa specifically recognized from 7 to 11 antigenic components of louse whole-body homogenates. Affinoblotting analysis with lectins showed that 10 of these components were glycoproteins with different types of glycan structure. Only one immunogenic antigen was isolated using immunoaffinity chromatography with the gammaglobulin fraction of an antigen-specific antiserum as immunospecific ligand. Further electrophoretic and immunochemical analyses revealed that it was a glycoprotein with relative molecular weight (RMW) 31 kDa and with high mannose type of oligosaccharide.
本文描述了刺多板虱蛋白质成分与抗原特异性大鼠抗血清以及五种植物凝集素的反应。在免疫印迹中，感染刺多板虱的大鼠血清能特异性识别虱全身体液匀浆中的7至11种抗原成分。凝集素亲和印迹分析表明，其中10种成分是具有不同聚糖结构类型的糖蛋白。以抗原特异性抗血清的γ球蛋白部分作为免疫特异性配体，通过免疫亲和层析仅分离出一种免疫原性抗原。进一步的电泳和免疫化学分析表明，它是一种相对分子质量为31 kDa的糖蛋白，具有高甘露糖型寡糖。
