Volf P, Grubhoffer L, Matha V
Department of Parasitology and Hydrobiology, Faculty of Sciences, Charles University, Prague, Czechoslovakia.
Folia Parasitol (Praha). 1990;37(3):275-8.
Number and relative molecular weights of proteins in whole body homogenates of the louse, Polyplax spinulosa were determined using SDS-PAGE. 13 protein components from the total of 28 bands were defined as Con A binding glycoproteins. In immunoblotting sera of lice infested laboratory rats of BN strain specifically recognized 3 major protein components with r.m.w. of 77,000, 105,000 and 230,000 Da and from 6 to 8 minor protein components with r.m.w. of 31,000-180,000 Da.
使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测定了多板刺虱（Polyplax spinulosa）全身匀浆中蛋白质的数量和相对分子量。在总共28条带中，有13种蛋白质成分被定义为刀豆球蛋白A（Con A）结合糖蛋白。在免疫印迹中，感染了虱子的BN品系实验大鼠血清特异性识别出3种主要蛋白质成分，相对分子质量分别为77,000、105,000和230,000道尔顿，以及6至8种次要蛋白质成分，相对分子质量为31,000 - 180,000道尔顿。
