Elgazzar R F, Mutabagani M A, Abdelaal S E, Sadakah A A
Faculty of Dentistry, Tanta University, Tanta, Egypt.
Int J Oral Maxillofac Surg. 2008 Aug;37(8):748-55. doi: 10.1016/j.ijom.2008.05.010. Epub 2008 Jun 25.
The aim of this study was to explore the ability of platelet rich plasma (PRP) to promote peripheral nerve regeneration after cyanoacrylate reanastomosis in rats. A total of 18 rats were used in this study. Bilateral sciatic neurotomies were performed in 15 rats, and then immediately reanastomosed with cyanoacrylate glue. On one side (G1), the anastomosed nerves were treated with prepared autologous PRP gel; on the contralateral side (G2) the nerves received no additional treatment. Sham surgery was undertaken on the remaining 3 rats (6 cases) where bilateral sciatic nerves were surgically approached but not cut (passive control group, (G3). Biopsies were harvested 12 weeks postoperatively and examined under the light microscope using osmic acid stain. The number of nerve fibers in the distal and proximal nerve segments of G1 and G2 as well as in G3 were counted and the results analyzed and compared. Animals in G1 and G2 showed some weakness and ulceration in their right and left feet for a few weeks postoperatively, which gradually improved during the follow-up period. The histomorphometric assessment showed a higher axon count in the distal segment of G1 (291.7 axons) compared with that of G2 (280.5 axons) (P=0.001). Similar results were noticed when the proximal segments of both groups were compared (P=0.040). These results were reflected in the values of the neurotization indices of G1 (91.9%) and G2 (89.5%) (P=0.008). The number of nerve fibers in G1 and G2 remained lower than in G3 (P=0.0001). The authors conclude that PRP may enhance the number of regenerating nerve fibers after cyanoacrylate neruoanastomosis.
本研究的目的是探讨富血小板血浆（PRP）促进大鼠氰基丙烯酸酯再吻合术后周围神经再生的能力。本研究共使用了18只大鼠。对15只大鼠进行双侧坐骨神经切断术，然后立即用氰基丙烯酸酯胶进行再吻合。在一侧（G1），用制备好的自体PRP凝胶处理吻合后的神经；在对侧（G2），神经不接受额外处理。对其余3只大鼠（6侧）进行假手术，手术暴露双侧坐骨神经但不切断（被动对照组，G3）。术后12周采集活检组织，用锇酸染色在光学显微镜下检查。对G1和G2以及G3的远端和近端神经节段中的神经纤维数量进行计数，并对结果进行分析和比较。G1和G2组的动物在术后几周内其左右脚出现了一些无力和溃疡情况，在随访期间逐渐改善。组织形态计量学评估显示，G1组远端节段的轴突计数（291.7个轴突）高于G2组（280.5个轴突）（P = 0.001）。比较两组近端节段时也观察到类似结果（P = 0.040）。这些结果反映在G1组（91.9%）和G2组（89.5%）的神经化指数值上（P = 0.008）。G1和G2组的神经纤维数量仍低于G3组（P = 0.0001）。作者得出结论，PRP可能会增加氰基丙烯酸酯神经吻合术后再生神经纤维的数量。
