[99mTc(PNP5)(DBODC)]+作为新型心肌灌注显像剂的实验研究

Experimental study of [99mTc(PNP5) (DBODC)]+ as a new myocardial perfusion imaging agent.

作者信息

Zhang Wan-Chun, Fang Wei, Li Bin, Wang Xue-Bin, He Zuo-Xiang

机构信息

Department of Nuclear Medicine, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Bei Li Shi Lu, Beijing, China.

出版信息

Cardiology. 2009;112(2):89-97. doi: 10.1159/000141013. Epub 2008 Jun 27.

DOI:10.1159/000141013

PMID:18583906

Abstract

OBJECTIVES

This study was aimed to compare the properties of [(99m)Tc(PNP5)(DBODC)]+ with (99m)Tc-MIBI in normal swine and in canine models of myocardial ischemia.

METHODS

Seven anesthetized swine were injected intravenously with [(99m)Tc(PNP5)(DBODC)]+. Serial planar images were acquired meanwhile. Twelve adult dogs were studied. A critical stenosis of left anterior descending artery was produced. [(99m)Tc(PNP5)(DBODC)]+ or (99m)Tc-MIBI (randomly) was injected intravenously at the end of 3 min of adenosine infusion. The occluder was released after adenosine infusion. Myocardial SPECT imaging was obtained at 30, 60, 90 and 180 min, respectively, after tracer injection. Rest myocardial SPECT imaging was acquired on the next day.

RESULTS

Blood disappearance of [(99m)Tc(PNP5)(DBODC)]+ was biexponential with an initial half-time of 2.97 +/- 0.48 min and a late half-time of 52.49 +/- 19.49 min. Myocardial [(99m)Tc(PNP5)(DBODC)]+ uptake was similar to (99m)Tc-MIBI. Heart-to-liver ratio of [(99m)Tc(PNP5)(DBODC)]+ was significantly higher than that of (99m)Tc-MIBI (0.99 +/- 0.03 vs. 0.46 +/- 0.10, p < 0.05) 30 min after injection. In canine models of myocardial ischemia, image quality of [(99m)Tc(PNP5)(DBODC)]+ was better than (99m)Tc-MIBI. Segments with perfusion defects by [(99m)Tc(PNP5)(DBODC)]+ was 3.60 +/- 1.52 and 4.25 +/- 0.96 by (99m)Tc-MIBI (p = n.s.).

CONCLUSIONS

[(99m)Tc(PNP5)(DBODC)]+ seems to be a promising new myocardial perfusion imaging agent.

摘要

目的

本研究旨在比较[(99m)Tc(PNP5)(DBODC)]+与(99m)Tc-MIBI在正常猪和犬心肌缺血模型中的特性。

方法

对7只麻醉的猪静脉注射[(99m)Tc(PNP5)(DBODC)]+。同时采集系列平面图像。对12只成年犬进行研究。制造左前降支严重狭窄。在腺苷输注3分钟结束时静脉注射[(99m)Tc(PNP5)(DBODC)]+或(99m)Tc-MIBI（随机）。腺苷输注后松开封堵器。分别在注射示踪剂后30、60、90和180分钟获得心肌SPECT图像。次日采集静息心肌SPECT图像。

结果

[(99m)Tc(PNP5)(DBODC)]+的血液清除呈双指数型，初始半衰期为2.97±0.48分钟，晚期半衰期为52.49±19.49分钟。心肌[(99m)Tc(PNP5)(DBODC)]+摄取与(99m)Tc-MIBI相似。注射后30分钟，[(99m)Tc(PNP5)(DBODC)]+的心脏与肝脏比值显著高于(99m)Tc-MIBI（0.99±0.03对0.46±0.10，p<0.05）。在犬心肌缺血模型中，[(99m)Tc(PNP5)(DBODC)]+的图像质量优于(99m)Tc-MIBI。[(99m)Tc(PNP5)(DBODC)]+显示灌注缺损的节段为3.60±1.52，(99m)Tc-MIBI为4.25±0.96（p=无显著差异）。