Lamsam Apipong, Laohaprapanon Sawanya, Annachhatre Ajit P
Environmental Engineering and Management, School of Environment, Resources and Development, Asian Institute of Technology, Khlong Luang, Pathumthani, Thailand.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2008 Aug;43(10):1198-208. doi: 10.1080/10934520802171766.
An activated sludge process with partial nitrification (AS/PN) in combination with anaerobic ammonium oxidation (Anammox) process for treatment of seafood processing wastewater was developed and investigated in this research. Operating conditions of AS/PN process for coupling with Anammox process were identified as pH between 7.7-8.2 and DO as 0.5-0.9 mg L(-1) to achieve over 85% COD removal as well as partial nitrification. The developed AS/PN process could produce almost equal concentration of ammonium and nitrite nitrogen in the effluent which was highly suitable for the Anammox process. Near complete removal of ammonium and nitrite was achieved during steady state Anammox process operation. Maximum nitrogen removal rate for the Anammox process was found to be 0.6 kg N m(- 3) d(-1). Microorganisms involved in both AS/PN and Anammox processes were identified using in situ hybridization and polymerase chain reaction techniques. The result 16S rDNA revealed 94% homology to Candidatus "Brocadia fulgida."
本研究开发并研究了一种采用部分硝化活性污泥工艺(AS/PN)与厌氧氨氧化(Anammox)工艺相结合的方法来处理海产品加工废水。确定了AS/PN工艺与Anammox工艺耦合的运行条件为pH值在7.7 - 8.2之间,溶解氧为0.5 - 0.9 mg L(-1),以实现超过85%的化学需氧量去除率以及部分硝化。所开发的AS/PN工艺能够在出水产生几乎等量浓度的铵态氮和亚硝酸盐氮,这非常适合Anammox工艺。在稳态Anammox工艺运行期间,铵态氮和亚硝酸盐几乎完全去除。发现Anammox工艺的最大氮去除率为0.6 kg N m(-3) d(-1)。使用原位杂交和聚合酶链反应技术鉴定了参与AS/PN和Anammox工艺的微生物。16S rDNA结果显示与“Candidatus Brocadia fulgida”具有94%的同源性。