Grarup Cecilie, Gleesen Ann Sofie, Dargis Rimtas, Andresen Keld, Christensen Jens Jørgen, Kemp Michael
Department of Bacteriology, Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark.
Scand J Infect Dis. 2008;40(6-7):547-50. doi: 10.1080/00365540701874426.
PCR using universal bacterial primers and subsequent DNA sequence analysis of the PCR product was used to detect beta-haemolytic streptococci in clinical samples. Streptococcus pyogenes, Streptococcus agalactiae and Streptococcus dysgalactiae were identified in 19 primary culture-negative cases of infections from 203 patient samples with detectable bacterial DNA.
使用通用细菌引物进行聚合酶链反应（PCR）并对PCR产物进行后续DNA序列分析，以检测临床样本中的β溶血性链球菌。在203份可检测到细菌DNA的患者样本中，19例原发性培养阴性感染病例中鉴定出化脓性链球菌、无乳链球菌和停乳链球菌。
