β-溶血性链球菌感染：通过通用PCR检测细菌DNA及DNA测序

Infections with beta-haemolytic streptococci: detection by a universal PCR for bacterial DNA and DNA sequencing.

作者信息

Grarup Cecilie, Gleesen Ann Sofie, Dargis Rimtas, Andresen Keld, Christensen Jens Jørgen, Kemp Michael

机构信息

Department of Bacteriology, Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark.

出版信息

Scand J Infect Dis. 2008;40(6-7):547-50. doi: 10.1080/00365540701874426.

DOI:10.1080/00365540701874426

PMID:18584545

Abstract

PCR using universal bacterial primers and subsequent DNA sequence analysis of the PCR product was used to detect beta-haemolytic streptococci in clinical samples. Streptococcus pyogenes, Streptococcus agalactiae and Streptococcus dysgalactiae were identified in 19 primary culture-negative cases of infections from 203 patient samples with detectable bacterial DNA.

摘要

使用通用细菌引物进行聚合酶链反应（PCR）并对PCR产物进行后续DNA序列分析，以检测临床样本中的β溶血性链球菌。在203份可检测到细菌DNA的患者样本中，19例原发性培养阴性感染病例中鉴定出化脓性链球菌、无乳链球菌和停乳链球菌。