Hetzler Laura E T, Sharma Nijee, Tanzer Lisa, Wurster Robert D, Leonetti John, Marzo Sam J, Jones Kathryn J, Foecking Eileen M
Department of Otolaryngology-Head & Neck Surgery, Loyola University Medical Center, Maywood, IL 60153, USA.
Otolaryngol Head Neck Surg. 2008 Jul;139(1):62-7. doi: 10.1016/j.otohns.2008.02.006.
We investigated the combined effects of electrical stimulation and testosterone propionate on overall recovery time in rats with extracranial crush injuries to the facial nerve.
Male rats underwent castration 3 to 5 days prior to right facial nerve crush injury and electrode implantation. Rats were randomly assigned to two groups: crush injury + testosterone or crush injury with electrical stimulation + testosterone. Recovery was assessed by daily subjective examination documenting vibrissae orientation/movement, semi-eye blink, and full eye blink.
Milestones of early recovery were noted to be significantly earlier in the groups with electrical stimulation, with/without testosterone. The addition of testosterone to electrical stimulation showed significant earlier return of late recovery parameters and complete overall recovery.
Electrical stimulation may decrease cell death or promote sprouting to accelerate early recovery. Testosterone may affect the actual rate of axonal regeneration and produce acceleration in functional recovery. By targeting different stages of neural regeneration, the synergy of electrical stimulation and testosterone appears to have promise as a neurotherapeutic strategy for facial nerve injury.
我们研究了电刺激和丙酸睾酮对颅外面神经挤压伤大鼠总体恢复时间的联合作用。
雄性大鼠在右侧面神经挤压伤和电极植入前3至5天进行去势。大鼠被随机分为两组：挤压伤+睾酮组或电刺激+挤压伤+睾酮组。通过每日主观检查记录触须方向/运动、半眨眼和全眨眼来评估恢复情况。
在有电刺激的组中，无论有无睾酮，早期恢复的里程碑均显著提前。电刺激联合睾酮显示晚期恢复参数的恢复显著提前，总体完全恢复。
电刺激可能减少细胞死亡或促进发芽以加速早期恢复。睾酮可能影响轴突再生的实际速率并加速功能恢复。通过针对神经再生的不同阶段，电刺激和睾酮的协同作用似乎有望成为面神经损伤的一种神经治疗策略。
