L-多巴脱羧酶（DDC）在前列腺癌中的表达分析及临床应用

Expression analysis and clinical utility of L-Dopa decarboxylase (DDC) in prostate cancer.

作者信息

Avgeris Margaritis, Koutalellis Georgios, Fragoulis Emmanuel G, Scorilas Andreas

机构信息

Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, Athens, Greece.

出版信息

Clin Biochem. 2008 Oct;41(14-15):1140-9. doi: 10.1016/j.clinbiochem.2008.04.026. Epub 2008 Jun 10.

DOI:10.1016/j.clinbiochem.2008.04.026

PMID:18586020

Abstract

BACKGROUND

L-Dopa decarboxylase (DDC) is a pyridoxal 5'-phosphate-dependent enzyme that was found to be involved in many malignancies. The aim of this study was to investigate the mRNA expression levels of DDC in prostate tissues and to evaluate its clinical utility in prostate cancer (CaP).

METHODS

Total RNA was isolated from 118 tissue specimens from benign prostate hyperplasia (BPH) and CaP patients and a highly sensitive quantitative real-time RT-PCR (qRT-PCR) method for DDC mRNA quantification has been developed using the SYBR Green chemistry. LNCaP prostate cancer cell line was used as a calibrator and GAPDH as a housekeeping gene.

RESULTS

DDC was found to be overexpressed, at the mRNA level, in the specimens from prostate cancer patients, in comparison to those from benign prostate hyperplasia patients (p<0.001). Logistic regression and ROC analysis have demonstrated that the DDC expression has significant discriminatory value between CaP and BPH (p<0.001). DDC expression status was compared with other established prognostic factors, in prostate cancer. High expression levels of DDC were found more frequently in high Gleason's score tumors (p=0.022) as well as in advanced stage patients (p=0.032).

CONCLUSIONS

Our data reveal the potential of DDC expression, at the mRNA level, as a novel biomarker in prostate cancer.

摘要

背景

L - 多巴脱羧酶（DDC）是一种依赖磷酸吡哆醛5'-磷酸的酶，已发现其与多种恶性肿瘤有关。本研究的目的是调查DDC在前列腺组织中的mRNA表达水平，并评估其在前列腺癌（CaP）中的临床应用价值。

方法

从良性前列腺增生（BPH）和CaP患者的118份组织标本中分离总RNA，并使用SYBR Green化学方法开发了一种用于DDC mRNA定量的高灵敏度定量实时RT - PCR（qRT - PCR）方法。以LNCaP前列腺癌细胞系作为校准物，以GAPDH作为管家基因。

结果

与良性前列腺增生患者的标本相比，发现前列腺癌患者标本中DDC在mRNA水平上过度表达（p<0.001）。逻辑回归和ROC分析表明，DDC表达在CaP和BPH之间具有显著的鉴别价值（p<0.001）。将DDC表达状态与前列腺癌中其他已确立的预后因素进行比较。在高Gleason评分肿瘤（p = 0.022）以及晚期患者（p = 0.032）中更频繁地发现DDC高表达水平。