Jain Monika, Ganju L, Katiyal A, Padwad Y, Mishra K P, Chanda S, Karan D, Yogendra K M S, Sawhney R C
Immunomodulation Laboratory, Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi 110054, India.
Phytomedicine. 2008 Oct;15(10):793-9. doi: 10.1016/j.phymed.2008.04.017. Epub 2008 Jun 30.
Dengue virus occurs as four distinct serotypes, called Dengue 1, 2, 3, and 4. Symptomatic dengue virus infection ranges from a self limited febrile illness, dengue fever (DF), to a more severe disease, dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). The anti-Dengue treatment is severely hampered as no specific therapeutic agents are available. Even present treatment strategies for Dengue are more supportive than curative. In the present study anti-dengue activity of Hippophae rhamnoides (Seabuckthorn, SBT) leaf extract was evaluated in Dengue virus type-2 infected blood-derived human macrophages as macrophages are the primary target of Dengue virus infection. Infected cells were treated with SBT leaf extract and compared with commercially available anti-viral drug, Ribavirin. The extract was able to maintain the cell viability of Dengue-infected cells at par with Ribavirin along with the decrease and increase in TNF-alpha and IFN-gamma respectively. Anti-dengue activity of SBT extract was further determined by the traditional plaque assay. These observations suggest that the SBT leaf extract has a significant anti-dengue activity and has the potential for the treatment of Dengue.
登革病毒有四种不同的血清型，分别称为登革1型、2型、3型和4型。有症状的登革病毒感染范围从自限性发热疾病登革热（DF）到更严重的疾病登革出血热/登革休克综合征（DHF/DSS）。由于没有可用的特异性治疗药物，登革热的治疗受到严重阻碍。即使是目前的登革热治疗策略也更多是支持性的而非治愈性的。在本研究中，沙棘（SBT）叶提取物在登革2型病毒感染的血液来源的人类巨噬细胞中评估其抗登革病毒活性，因为巨噬细胞是登革病毒感染的主要靶点。用SBT叶提取物处理感染细胞，并与市售抗病毒药物利巴韦林进行比较。该提取物能够使登革热感染细胞的细胞活力与利巴韦林相当，同时分别降低和升高肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）。通过传统的蚀斑测定进一步确定SBT提取物的抗登革病毒活性。这些观察结果表明，SBT叶提取物具有显著的抗登革病毒活性，具有治疗登革热的潜力。
