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沙棘叶提取物对白化大鼠皮肤伤口愈合影响的临床前研究。

A preclinical study of the effects of seabuckthorn (Hippophae rhamnoides L.) leaf extract on cutaneous wound healing in albino rats.

作者信息

Gupta Asheesh, Kumar Ratan, Pal Karan, Banerjee Pratul K, Sawhney Ramesh C

机构信息

Defence Institute of Physiology and Allied Sciences, Delhi, India.

出版信息

Int J Low Extrem Wounds. 2005 Jun;4(2):88-92. doi: 10.1177/1534734605277401.

Abstract

Hippophae rhamnoides L. (family Elaeagnaceae), commonly known as seabuckthorn, is a wild shrub growing at high altitude (1200-4500 meters) in adverse climatic conditions. The aim of the present study was to evaluate healing potential of seabuckthorn leaves in a preclinical study on rats using a cutaneous excision-punch wound model. Four full-thickness excision-type wounds of 8.0 mm diameter were created on the dorsal surface of rats under aseptic conditions. The aqueous lyophilized extract of seabuckthorn leaves, at doses of 0.5%, 1.0%, and 1.5% w/v prepared in propylene glycol, were applied topically twice daily for 7 days. Control animals received the vehicle alone in an identical manner. Wound granulation tissues were excised on eighth day postwounding, and the hydroxyproline, hexosamine, total protein content, and antioxidant levels were determined. Wound surface area was also measured on the eighth day before wound excision to determine wound contraction. Topical application of 1.0% seabuckthorn leaf extract statistically significantly augmented the healing process, as evidenced by increases in the content of hydroxyproline and protein as well as the reduction in wound area when compared with similar effects in response to treatment using povidone-iodine ointment (standard care). The reduced glutathione, vitamin C, superoxide dismutase, catalase, and glutathione peroxidase activities showed significant increases in seabuckthorn leaf extract-treated wounds as compared to controls. The lipid peroxide levels were significantly decreased in leaf extract-treated wounds. The results suggest that aqueous leaf extract of seabuckthorn promotes wound healing, which may be due to increased antioxidant levels in the granulation tissue.

摘要

沙棘(胡颓子科),通常被称为醋柳,是一种生长在高海拔(1200 - 4500米)恶劣气候条件下的野生灌木。本研究的目的是在大鼠的临床前研究中,使用皮肤切除打孔伤口模型评估沙棘叶的愈合潜力。在无菌条件下,于大鼠背部制造4个直径8.0毫米的全层切除型伤口。将沙棘叶的水冻干提取物,以0.5%、1.0%和1.5%(w/v)的剂量用丙二醇配制,每天局部涂抹两次,持续7天。对照动物以相同方式仅接受赋形剂。在伤口形成后第8天切除伤口肉芽组织,并测定羟脯氨酸、氨基己糖、总蛋白含量和抗氧化水平。在切除伤口前的第8天也测量伤口表面积,以确定伤口收缩情况。与使用聚维酮碘软膏(标准护理)治疗的类似效果相比,局部应用1.0%沙棘叶提取物在统计学上显著增强了愈合过程,表现为羟脯氨酸和蛋白质含量增加以及伤口面积减小。与对照组相比,沙棘叶提取物处理的伤口中还原型谷胱甘肽、维生素C、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性显著增加。叶提取物处理的伤口中脂质过氧化物水平显著降低。结果表明,沙棘叶水提取物促进伤口愈合,这可能是由于肉芽组织中抗氧化水平增加所致。

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