变性梯度凝胶电泳作为一种确定猪盲肠细菌益生菌培养物批次相似性的工具。
Denaturing gradient gel electrophoresis as a tool to determine batch similarity of probiotic cultures of porcine cecal bacteria.
作者信息
Hume Michael E, Scanlan Charles M, Harvey Roger B, Andrews Kathleen, Snodgrass James D, Nalian Armen G, Martynova-Van Kley Alexandra, Nisbet David J
机构信息
USDA, ARS, SPARC, FFSRU, 2881 F&B Road, College Station, TX 77845, USA.
出版信息
Appl Environ Microbiol. 2008 Aug;74(16):5241-3. doi: 10.1128/AEM.02580-07. Epub 2008 Jun 27.
Abstract
A continuous-flow porcine cecal bacterial culture has been used experimentally as treatment against enterotoxigenic Escherichia coli in weanling pigs. Periodically, the cultures must be started from frozen stock. Our results indicate that denaturing gradient gel electrophoresis can be applied as an indirect indication of culture similarity for each new batch generated from frozen stock.
摘要
连续流猪盲肠细菌培养物已被用于仔猪抗产肠毒素大肠杆菌的实验性治疗。培养物必须定期从冷冻菌种开始。我们的结果表明，变性梯度凝胶电泳可作为从冷冻菌种产生的每一批新培养物培养相似性的间接指标。
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