Sada E, Katoh S, Miyoshi H, Yamanaka K, Kondo A
Chemical Engineering Department, Kyoto University, Kyoto 606, Japan.
Biotechnol Bioeng. 1988 Aug 5;32(4):467-74. doi: 10.1002/bit.260320409.
Antisera against charged (p-azobenzoate and p-azoben zenesulfonate) and uncharged (dinitrophenyl) haptenic groups were produced in rabbits, and the equilibrium characteristics of hapten-antibody were measured by use of immunoadsorbents. The antibody to the uncharged hapten formed a stable binding with the hapten to the changes in ionic strength and pH. On the other hand, the antibodies to the charged haptens showed affinities sensitive to the changes in pH and ionic strength. Therefore, the effect of the pK(a) of ionizable haptens on the pH dependence of the hapten-antibody binding was studied by comparing the interactions between a series of para-substituted benzoic acids and the anti-p-azobenzoate antibody. The pH dependence of the interactions was strongly affected by the pK(a) of ionizable groups in haptens. Furthermore, the equilibrium characteristics of anti-p-aminobenzoyl dipeptides were compared. The characteristics of interactions were affected by the features of amino acid residues.
在兔子体内制备了针对带电（对氨基苯甲酸盐和对氨基苯磺酸酯）和不带电（二硝基苯基）半抗原基团的抗血清，并使用免疫吸附剂测量了半抗原 - 抗体的平衡特性。针对不带电半抗原的抗体与半抗原形成了稳定的结合，不受离子强度和pH变化的影响。另一方面，针对带电半抗原的抗体表现出对pH和离子强度变化敏感的亲和力。因此，通过比较一系列对取代苯甲酸与抗对氨基苯甲酸抗体之间的相互作用，研究了可电离半抗原的pK(a)对半抗原 - 抗体结合的pH依赖性的影响。相互作用的pH依赖性受到半抗原中可电离基团的pK(a)的强烈影响。此外，还比较了抗对氨基苯甲酰二肽的平衡特性。相互作用的特性受氨基酸残基特征的影响。
