Brient B W, Nisonoff A
J Exp Med. 1970 Nov;132(5):951-62. doi: 10.1084/jem.132.5.951.
Rabbit anti-idiotypic antibodies were prepared by injection of specifically purified anti-p-azobenzoate antibodies (D) from individual donor rabbits. Benzoate derivatives were found to be strong inhibitors of the reactions of D with anti-D antisera. There was a close correlation between the combining affinities of the benzoate derivatives used and their effectiveness as inhibitors. Compounds tested that are chemically unrelated to benzoate were ineffective. The results indicate either that the combining site of anti-benzoate antibody is part of an important idiotypic determinant, which is sterically blocked by hapten, or that the hapten induces a conformational change which alters idiotypic determinants not involving the active site. Such conformational changes, if they occur, must be restricted since hapten has little effect on the reactions of F(ab')(2) fragments of anti-benzoate antibodies with antisera directed to rabbit fragment Fab and no detectable effect on reactions with antibodies directed to allotypic determinants.
通过向个体供体兔注射特异性纯化的抗对氨基苯甲酸酯抗体（D）来制备兔抗独特型抗体。发现苯甲酸酯衍生物是D与抗D抗血清反应的强抑制剂。所用苯甲酸酯衍生物的结合亲和力与其作为抑制剂的有效性之间存在密切相关性。测试的与苯甲酸酯化学无关的化合物无效。结果表明，抗苯甲酸酯抗体的结合位点要么是重要独特型决定簇的一部分，该决定簇在空间上被半抗原阻断，要么是半抗原诱导了构象变化，从而改变了不涉及活性位点的独特型决定簇。如果发生这种构象变化，必然是受到限制的，因为半抗原对抗苯甲酸酯抗体的F(ab')(2)片段与针对兔Fab片段的抗血清的反应几乎没有影响，对与针对同种异型决定簇的抗体的反应也没有可检测到的影响。
