Kimura T, Ogata M, Yoshida M, Nakakuki T
Engineering and Development Division, Chiyoda Corporation, Yokohama 213, Japan.
Biotechnol Bioeng. 1988 Aug 20;32(5):669-76. doi: 10.1002/bit.260320512.
A continuous production process of maltotetraose was investigated by using immobilized maltotetraose (G(4))- forming amylase (1,4-alpha-D-glucan maltotetraohydrolase, EC3.2.1.60) from Pseudomonas stutzeri adsorbed on a macroporous hydrophobic resin. The maximum reaction rate was obtained at 55 degrees C and the activation energy of hydrolysis by immobilized G(4)-forming amylase was calculated to be 8.45 kcal/mol. The maltotetraose yield was greatly influenced by the flow rate of substrate solution, its concentration, and the immobilized enzyme activity. The newly defined factor "specific space velocity" was successfully introduced to normalize the operating parameters. Using this factor, the immobilized enzyme reactor then can be simulated and the operating dynamics can be determined.
利用吸附在大孔疏水树脂上的来自施氏假单胞菌的固定化麦芽四糖（G(4)）生成淀粉酶（1,4-α-D-葡聚糖麦芽四糖水解酶，EC3.2.1.60），对麦芽四糖的连续生产工艺进行了研究。在55℃时获得最大反应速率，经计算固定化G(4)生成淀粉酶的水解活化能为8.45千卡/摩尔。麦芽四糖的产量受底物溶液流速、其浓度以及固定化酶活性的极大影响。成功引入新定义的“比空间速度”因子来规范操作参数。利用该因子，随后可以模拟固定化酶反应器并确定操作动力学。
