Department of Chemical Engineering, University of California, Berkeley, California 94720, USA.
Biotechnol Bioeng. 1989 Jan 20;33(4):487-99. doi: 10.1002/bit.260330414.
The transient and steady-state responses of hybridoma growth and metabolism to glutamine pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The specific glutamine consumption rate increased rapidly after all glutamine additions, but the responses of the glucose and oxygen consumption rates and the cell concentration were found to depend on the intial feed glutamine concentration. The glucose consumption rate was 1.4-10.9 times that of glutamine, and serine and branched-chain amino acids were consumed in larger amounts at the higher glucose: glutamine uptake ratios. It was estimated that maintenance accounted for ca. 60% of the cellular ATP requirements at specific growth rates ranging from 0.57 to 0.68 day(-1).
杂交瘤生长和代谢对谷氨酰胺脉冲和阶跃变化的瞬态和稳态响应已经过检验。报告了氧、葡萄糖、乳酸、氨、谷氨酰胺、丙氨酸和其他氨基酸的代谢系数。所有谷氨酰胺添加后，特异性谷氨酰胺消耗率迅速增加，但葡萄糖和氧气消耗率以及细胞浓度的响应取决于初始进料谷氨酰胺浓度。葡萄糖消耗率是谷氨酰胺的 1.4-10.9 倍，并且在较高的葡萄糖：谷氨酰胺摄取比下，丝氨酸和支链氨基酸的消耗更大。据估计，在特定生长速率为 0.57 至 0.68 天(-1)的范围内，维持约占细胞 ATP 需求的 60%。
