Franĕk F, Dolníková J
Department of Fundamental Cytotechnology, Czechoslovak Academy of Sciences, Praha.
Cytotechnology. 1991 Sep;7(1):33-8. doi: 10.1007/BF00135636.
The iron-rich (500 microM ferric citrate) protein-free supplement was added to six different basal media. Cell growth and monoclonal antibody production of a mouse-mouse hybridoma were investigated in 1.3 1 batch cultures performed in a laboratory bioreactor with automatic control of pH and dissolved oxygen concentration. RPMI 1640 served as the control medium. Fortification of the basal medium by balanced mixtures of amino acids and vitamins showed higher positive effect than daily supplementation by glucose and glutamine. Strongly fortified medium, based on RPMI 1640, was found superior to other basal media. The viability index increased by a factor of 3.04 and the total antibody production by a factor of 2.82, relative to the control.
将富含铁（500微摩尔柠檬酸铁）的无蛋白补充剂添加到六种不同的基础培养基中。在实验室生物反应器中进行的1.3升分批培养中，研究了小鼠-小鼠杂交瘤的细胞生长和单克隆抗体产生情况，该生物反应器可自动控制pH值和溶解氧浓度。RPMI 1640用作对照培养基。与每日补充葡萄糖和谷氨酰胺相比，用氨基酸和维生素的平衡混合物强化基础培养基显示出更高的积极效果。基于RPMI 1640的强化程度高的培养基被发现优于其他基础培养基。相对于对照，活力指数提高了3.04倍，总抗体产量提高了2.82倍。
