Zhang Meiyun, Cheng Yibin, Dong Shaoxia, Liu Ya
Institute for Environmental Health and Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Wei Sheng Yan Jiu. 2008 Mar;37(2):155-8.
Objective Based on the distribution of genetic polymorphisms of GSTM1 ,GSTT1 and CYP1A1 genotypes in mothers and neonates with preterm delivery and health controls, to explore the relationship between these gene polymorphisms and susceptibility to preterm delivery.
we investigated mothers and neonates with preterm delivery and health controls were investigated by case-control method. The frequencies of GSTM1, GSTr1 and CYP1A1 genotypes were detected by PCR and RFLP-PCR techniques.
The difference of variation frequencies of GSTM1, GSTT1 and CYP1A1 (Msp I polymorphism) genotypes between cases (mothers and neonates respectively) and their health controls were not statistically significant (P > 0.05). The difference of variation frequencies of GSTM1 and GSTT1 combined between mother cases group and control groups were not statistically significant (P > 0.05). The neonates was same as mother groups. However, the differences of CYP1A1 heterozygous mutation and homozygous mutation gene combined with the null genotypes among GSTT1 between mother groups with preterm delivery and health controls were statistically significant (chi2 = 4.683, P < 0.05, OR = 2.440).
CYP1A1 heterozygous mutation and homozygous mutation gene combined with GSTT1 null genotypes in mothers could increase the risk of preterm delivery.
基于早产母亲及新生儿与健康对照者中谷胱甘肽硫转移酶M1（GSTM1）、谷胱甘肽硫转移酶T1（GSTT1）和细胞色素P450 1A1（CYP1A1）基因多态性的分布情况，探讨这些基因多态性与早产易感性之间的关系。
采用病例对照研究方法，对早产母亲及新生儿与健康对照者进行调查。采用聚合酶链反应（PCR）和限制性片段长度多态性聚合酶链反应（RFLP-PCR）技术检测GSTM1、GSTT1和CYP1A1基因型频率。
病例组（分别为母亲和新生儿）与其健康对照者之间GSTM1、GSTT1和CYP1A1（Msp I多态性）基因型变异频率的差异无统计学意义（P>0.05）。母亲病例组与对照组之间GSTM1和GSTT1联合变异频率的差异无统计学意义（P>0.05）。新生儿情况与母亲组相同。然而，早产母亲组与健康对照者之间，CYP1A1杂合突变和纯合突变基因与GSTT1无效基因型联合的差异有统计学意义（χ2=4.683，P<0.05，OR=2.440）。
母亲中CYP1A1杂合突变和纯合突变基因与GSTT1无效基因型联合可增加早产风险。
