Rossi G A, Crimi E, Lantero S, Gianiorio P, Oddera S, Crimi P, Brusasco V
First Division of Pneumonology, Hospital San Martino, School of Physiopathology, University of Genoa, Italy.
Am Rev Respir Dis. 1991 Aug;144(2):379-83. doi: 10.1164/ajrccm/144.2.379.
To determine whether a link exists between the recruitment of inflammatory cells in the airways and the development of the late-phase asthmatic reaction, we studied with bronchoalveolar lavage 54 asthmatic patients either at baseline (10 patients) or 4 h (11 patients), 24 h (13 patients), and 72 h (20 patients) after allergen inhalation challenge. Among the patients studied 4 h after allergen challenge, five were known to have a late-phase asthmatic response and showed a significant increase in the number and percentage of eosinophils in bronchoalveolar lavage compared with either patients without late-phase response (p less than 0.05) or unchallenged patients (p less than 0.01). Both the number and the percentage of eosinophils in bronchoalveolar lavage were also increased (p less than 0.05) in patients without a late-phase asthmatic reaction studied 24 h but not in those studied 4 h after allergen challenge. The numbers and the percentages of macrophages, neutrophils, or lymphocytes did not differ significantly among the different groups of patients. Of the patients studied 4 and 24 h after allergen challenge, only those with a late-phase asthmatic response showed an increased airway responsiveness to methacholine 1 h before bronchoalveolar lavage. We conclude that the development of the late-phase asthmatic response to allergen inhalation challenge and the allergen-induced increase in airway responsiveness are associated with an early recruitment of eosinophils in the airways.
为了确定气道中炎症细胞的募集与迟发性哮喘反应的发生之间是否存在联系，我们对54例哮喘患者进行了支气管肺泡灌洗研究，这些患者分别处于基线状态（10例）或在变应原吸入激发后4小时（11例）、24小时（13例）和72小时（20例）。在变应原激发后4小时进行研究的患者中，已知有5例发生迟发性哮喘反应，与无迟发性反应的患者（p<0.05）或未激发的患者（p<0.01）相比，其支气管肺泡灌洗中嗜酸性粒细胞的数量和百分比显著增加。在变应原激发后24小时进行研究但无迟发性哮喘反应的患者中，支气管肺泡灌洗中嗜酸性粒细胞的数量和百分比也增加了（p<0.05），但在变应原激发后4小时进行研究的患者中则没有增加。不同组患者之间巨噬细胞、中性粒细胞或淋巴细胞的数量和百分比没有显著差异。在变应原激发后4小时和24小时进行研究的患者中，只有那些有迟发性哮喘反应的患者在支气管肺泡灌洗前1小时对乙酰甲胆碱的气道反应性增加。我们得出结论，对变应原吸入激发的迟发性哮喘反应的发生以及变应原诱导的气道反应性增加与气道中嗜酸性粒细胞的早期募集有关。
