Mady Mohsen M, Ghannam M M, Khalil W A, Müller R, Fahr Alfred
Department of Biophysics, Faculty of Science, Cairo University, 12613 Giza, Egypt.
Phys Med. 2009 Jun;25(2):88-93. doi: 10.1016/j.ejmp.2008.05.005. Epub 2008 Jun 30.
It is necessary to understand liposomal uptake mechanisms and intracellular distribution in order to design more efficient gene (drug) carrier systems. Until now, a few studies have been carried out using confocal laser scanning microscopy (CLSM) to investigate the cellular uptake and transfection mediated with liposomes. So, by CLSM, we demonstrated that artificial virus-like envelope (AVE) vesicles labeled with rhodamine-PE (Rh-PE), carbocyanine (DiI) and carboxyfluorescein (CF) were investigated into the cytoplasm of two human cell lines, Mewo (human melanoma cell line) and HepG2 (human hepatoma cell line) cells grown in DMEM medium supplemented with different percentages (0%, 30%, and 100%) fetal calf serum (FCS). The liposome uptake was dependent on the cell line, in view that the whole process of liposomes associated with cells (uptake) is a two-step process involving binding and endocytosis. Based upon the various assays used to measure cellular uptake of liposomes, we conclude the efficacy of cytoplasmic delivery by AVE-liposomes to cells in culture.
为了设计更高效的基因（药物）载体系统，了解脂质体摄取机制和细胞内分布是很有必要的。到目前为止，已经开展了一些使用共聚焦激光扫描显微镜（CLSM）来研究脂质体介导的细胞摄取和转染的研究。因此，通过CLSM，我们证明了用罗丹明-PE（Rh-PE）、羰花青（DiI）和羧基荧光素（CF）标记的人工病毒样包膜（AVE）囊泡被研究进入在补充有不同百分比（0%、30%和100%）胎牛血清（FCS）的DMEM培养基中生长的两种人类细胞系，即Mewo（人类黑色素瘤细胞系）和HepG2（人类肝癌细胞系）细胞的细胞质中。鉴于脂质体与细胞相关的整个过程（摄取）是一个涉及结合和内吞作用的两步过程，脂质体的摄取取决于细胞系。基于用于测量脂质体细胞摄取的各种测定方法，我们得出AVE-脂质体向培养细胞进行细胞质递送的效果。
