Schaeffer-Reiss Christine
Laboratoire de Spectrométrie de Masse Bio-Organique, Institut Pluridisciplinaire Hubert Curien, UMR 7178 CNRS/ Université Louis Pasteur, Strasbourg, France.
Methods Mol Biol. 2008;484:3-16. doi: 10.1007/978-1-59745-398-1_1.
Recent technical innovations in mass spectrometry-based techniques have resulted in a range of highly sensitive and versatile instruments for high-throughput, high-sensitive, proteome-scale profiling. This wide diversity of instrumentation commercially available for mass spectrometry-based proteomics makes the choice of instrumentation sometimes difficult. The choice of instruments depends on the biological problem and the proteomic strategy chosen for protein identification. This chapter will give a short overview of the instruments routinely used in proteomic laboratories and the technical criteria that should be considered before instrument selection.
基于质谱技术的近期技术创新带来了一系列用于高通量、高灵敏度蛋白质组规模分析的高灵敏度且通用的仪器。市售的用于基于质谱的蛋白质组学的仪器种类繁多,这使得仪器的选择有时颇具难度。仪器的选择取决于生物学问题以及为蛋白质鉴定所选用的蛋白质组学策略。本章将简要概述蛋白质组学实验室中常规使用的仪器以及在选择仪器之前应考虑的技术标准。