Gelderman Monique P, Simak Jan
Laboratory of Cellular Hematology, CBER FDA, Rockville, MD, USA.
Methods Mol Biol. 2008;484:79-93. doi: 10.1007/978-1-59745-398-1_6.
Cell membrane microparticles (MPs) are phospholipid microvesicles shed from the plasma membrane of most eukaryotic cells undergoing activation or apoptosis. The presence of MPs is common in healthy individuals. However, an increase in their release is a controlled event and is considered a hallmark of cellular alteration. Microparticles display cell surface proteins that indicate their cellular origin. In addition, they may also express other markers, e.g., markers of cellular activation. Elevated levels of circulating MPs are associated with various vascular pathologies and their pathogenic potential has been widely documented. MPs have been analyzed in plasma and cell cultures by means of flow cytometry or solid phase assays. Here we present a three-color flow cytometric assay for immunophenotyping of MPs in plasma. This assay has been used to study elevated counts of different phenotypes of circulating endothelial MPs in several hematological and vascular diseases. A modified version of this assay can also be used for MP analysis in blood products and cell cultures.
细胞膜微粒（MPs）是从大多数经历激活或凋亡的真核细胞膜上脱落的磷脂微囊泡。在健康个体中，MPs的存在很常见。然而，其释放量的增加是一个可控事件，被认为是细胞改变的标志。微粒展示出表明其细胞来源的细胞表面蛋白。此外，它们还可能表达其他标志物，例如细胞激活标志物。循环MPs水平升高与多种血管病变相关，其致病潜力已得到广泛记录。已通过流式细胞术或固相测定法在血浆和细胞培养物中分析MPs。在此，我们介绍一种用于血浆中MPs免疫表型分析的三色流式细胞术检测方法。该检测方法已用于研究多种血液学和血管疾病中循环内皮MPs不同表型的计数升高情况。此检测方法的改良版本也可用于血液制品和细胞培养物中的MP分析。
